cis-Diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific.
cis-Diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific.
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顺式二氯二氨铂介导的核蛋白与 DNA 的交联是细胞周期特异性的。
DOI：
10.1016/0003-9861(85)90270-x
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发表时间：
1985
影响因子：
3.9
通讯作者：
Stein,GS
中科院分区：
文献类型：
--
作者：
Banjar,ZM;Hnilica,LS;Briggs,RC;Dominguez,E;Stein,JL;Stein,GS
应用免疫组化方法研究顺铂（cis-DDP）对HeLa-S_3细胞周期依赖性蛋白质-DNA交联的影响。用双胸苷阻断或羟基脲同步化的细胞，在经过S_1、G_2、M期，然后进入G_1和S期的过程中，每隔2 h用顺式顺铂孵育。DNA交联抗原与抗血清反应，以0.35mNaCl提取物或残留的HeLa S期细胞核的免疫印迹显示，几个抗原改变了他们的DNA交联模式的HeLa细胞的进展过程中，通过其生殖周期。
Immunochemical analysis was employed to investigate the cell cycle-dependent protein-DNA crosslinking bycis-diamminedichloroplatinum II (cis-DDP), in HeLa-S3cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals withcis-DDP as they progressed through S1, G2, M, and then into G1and S phases of the subsequent cycle. Immunoblots of the DNA-crosslinked antigens reacted with antisera to 0.35mNaCl extract or residue of HeLa S-phase nuclei revealed that several antigens changed their DNA-crosslinking pattern during the progression of HeLa cells through their reproductive cycle.