The role of ABI3 and FUS3 loci in Arabidopsis thaliana on phase transition from late embryo development to germination
The role of ABI3 and FUS3 loci in Arabidopsis thaliana on phase transition from late embryo development to germination
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DOI：
10.1006/dbio.2000.9632
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发表时间：
2000-04-15
影响因子：
2.7
通讯作者：
Naito, S
中科院分区：
文献类型：
--
作者：
Nambara, E;Hayama, R;Naito, S
拟南芥ABI3和FUS3突变体在胚胎发育后期存在缺陷，胚胎表现出早熟生长。为了了解ABI3和FUSS的功能和作用，我们分析了这些突变体在胚胎发育后期和萌发过程中正常激活的基因的表达模式。利用差异显示技术，在ABI3fus3双突变体的未成熟角果中观察到上调和下调的基因。分离到4个在ABI3fus3双突变体中比野生型表达更丰富的克隆。这些基因在野生型萌发过程中被激活，这表明在野生型萌发过程中被激活的一些基因在ABI3融合3突变体的胚胎发育后期被提前激活。此外，还分离了在野生型萌发过程中被激活的基因，并分析了它们在野生型和abi3、fus3和abi3fus3突变体胚胎发育后期的表达模式。发现了16个这样的克隆，其中II个在突变体中表现出基因表达的去抑制或早熟激活。这些结果表明，ABI3和FUSS在胚胎发育后期负调控一组特定的基因。我们还发现，未成熟的Fus3角果积累的脱落酸(AFA)是野生型的三分之一，而成熟的Fus3角果积累的ABA水平与野生型相当。讨论了在胚胎发育后期控制发育时机的可能机制，以及ABI3和FUSS的协同和不同作用。(C)2000年学术出版社。
Arabidopsis abi3 and fus3 mutants are defective in late embryo development and their embryos show precocious growth. To understand the function and role of ABI3 and FUSS, we analyzed expression patterns of genes which were normally activated during late embryo development and germination in these mutants. Using the differential display method, both upregulated and downregulated genes were observed in immature siliques of the abi3 fus3 double mutant. Four clones having more abundant expression in the abi3 fus3 double mutant than in wild type were isolated. These genes were activated during wild-type germination, suggesting that some genes that are activated during wild type germination are precociously activated in the abi3 fus3 mutant during late embryo development. Also, genes that were activated during wild-type germination were isolated and their expression patterns during late embryo development in the wild type and in abi3, fus3, and abi3 fus3 mutants were analyzed. Sixteen such clones were found, and II of these showed derepression or precocious activation of gene expression in the mutants. These results indicate that ABI3 and FUSS negatively regulate a particular set of genes during late embryo development. We also showed that immature fus3 siliques accumulated one-third of the wild-type level of abscisic acid (AFA), but mature fus3 siliques accumulated ABA at a level comparable to that in the wild type. The possible mechanisms of controlling developmental timing in late embryo development as well as collaborative and distinct roles of ABI3 and FUSS are discussed. (C) 2000 Academic Press.