Human histo-blood group ABO glycosyltransferase genes: different enhancer structures with different transcriptional activities.
Human histo-blood group ABO glycosyltransferase genes: different enhancer structures with different transcriptional activities.
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DOI：
10.1006/bbrc.2000.2962
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发表时间：
2000-07
影响因子：
3.1
通讯作者：
L. C. Yu;C. Chang;Y. Twu;M. Lin
中科院分区：
文献类型：
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作者：
L. C. Yu;C. Chang;Y. Twu;M. Lin
人类组织血型ABO糖基转移酶基因的增强子元件位于转录起始点的上游-3.7kb，由一个43bp的单位的四个串联重复组成。最近我们在等位基因A、B和O糖基转移酶基因中发现了三种不同的增强子结构。该增强子结构有4个43个碱基，存在于B和O基因中，而不存在于A基因中。A基因相应的增强子区域只包含一个43bp的单位，在这个单位内，当与共同序列比较时，存在核苷酸替代。瞬时转染实验表明，A基因增强子区域的转录活性不到B基因增强子的1%。当与ABO基因的天然启动子协同作用时，两个增强子的转录活性之间的差异变得更加显著。这两个等位基因的43个碱基的重复数不同是导致A基因和B基因增强子转录活性差异的主要原因。
The enhancer element of the human histo-blood group ABO glycosyltransferase gene has been demonstrated to be located -3.7 kb upstream from the transcription start site and to be composed of four tandem repeats of a 43-bp unit. Recently we identified three different enhancer structures among the allelic A, B, and O glycosyltransferase genes. The enhancer structure with four 43-bp units is present in the B and O genes, but not in the A gene. The corresponding enhancer region of the A gene contains only one 43-bp unit, and within this unit a nucleotide substitution exists when compared with the consensus sequence. Through transient transfection assays, the transcriptional activity of the A-gene enhancer region was demonstrated to be less than 1% of that of the B-gene enhancer. The difference between the transcriptional activities of the two enhancers became more significant when acting in concert with the ABO-gene's native promoter. The different repeat numbers of the 43-bp unit possessed by the two allelic genes were shown to be the main reason for the vast difference in the transcriptional activities between the A-gene and B-gene enhancers.