Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons.
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons.
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DOI：
10.7554/elife.71752
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发表时间：
2021-11-26
期刊：
影响因子：
7.7
通讯作者：
Davidson S
中科院分区：
文献类型：
--
作者：
Nguyen MQ;von Buchholtz LJ;Reker AN;Ryba NJ;Davidson S
背根神经节（DRG）中具有胞体的体感神经元投射到皮肤、肌肉、骨骼和内脏，以检测触觉和温度，以及介导本体感觉和许多类型的内感觉。此外，躯体感觉系统传递临床相关的疼痛和瘙痒的有害感觉。在这里，我们使用单核转录组学来表征检测这些不同类型刺激的人类DRG神经元的转录组学类别。值得注意的是，多种类型的人类DRG神经元具有类似于其小鼠对应物的转录组学特征，尽管被认为对感觉功能重要的基因的表达在物种之间通常不同。更出乎意料的是，我们确定了几个转录组类，在其他物种中没有明确的等价物。该数据集应该作为社区的宝贵资源，例如作为将翻译工作集中在跨物种保守表达的分子上的手段。
Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to the skin, muscles, bones, and viscera to detect touch and temperature as well as to mediate proprioception and many types of interoception. In addition, the somatosensory system conveys the clinically relevant noxious sensations of pain and itch. Here, we used single nuclear transcriptomics to characterize transcriptomic classes of human DRG neurons that detect these diverse types of stimuli. Notably, multiple types of human DRG neurons have transcriptomic features that resemble their mouse counterparts although expression of genes considered important for sensory function often differed between species. More unexpectedly, we identified several transcriptomic classes with no clear equivalent in the other species. This dataset should serve as a valuable resource for the community, for example as means of focusing translational efforts on molecules with conserved expression across species.