Human parvovirus B19 infection of monocytic cell line U937 and antibody-dependent enhancement
Human parvovirus B19 infection of monocytic cell line U937 and antibody-dependent enhancement
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DOI：
10.1016/j.virol.2005.09.040
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发表时间：
2006-02-05
期刊：
影响因子：
3.7
通讯作者：
Sasaki, T
中科院分区：
文献类型：
--
作者：
Munakata, Y;Kato, I;Sasaki, T
人细小病毒B19（B19）感染人红系细胞。越来越多的证据也表明，B 19在体内非红系细胞中是可检测的，但感染的机制仍不清楚。本研究探讨了B19对人单核细胞系U937的感染方式。体外感染研究证明B19结合U937，B19-DNA复制缓慢，B19-NS 1 mRNA转录。B19-DNA在U937细胞中的复制伴随着B19-VP 1 mRNA的转录水平检测不到，表明B19感染U937细胞可能是失败的。B19-DNA和B19-NS 1 mRNA转录水平在抗B19 IgG抗体存在下增加，但这种作用在抗Fc受体抗体存在下降低，显示B19感染的抗体依赖性增强。抗体依赖性增强也导致U937中TNF α的产生增加。这项研究首次提出B19感染非红系细胞具有抗体依赖性增强作用。(C)2005年爱思唯尔公司All rights reserved.
Human parvovirus B19 (B19) infects human erythroid lineage cells. Accumulating evidence also shows that B 19 is detectable in nonerythroid lineage cells in vivo, but the mechanism of infection is still not clear. In this study, we explored the mode of B19 infection of human monocytic cell line U937. An in vitro infection study demonstrated B19 binding of U937 and slow replication of B19-DNA with B19-NS1 mRNA transcription. B19-DNA replication in U937 was accompanied by undetectable level of B19-VP1 mRNA transcription, indicating that B19 infection of U937 cells may be abortive. Levels of B19-DNA and B19-NS1 mRNA transcription increased in the presence of anti-B19 IgG antibodies, but this effect decreased in the presence of anti-Fc receptor antibodies, showing antibody-dependent enhancement by B19 infection. Antibody-dependent enhancement also caused the increased production of TNF alpha in U937. This study is the first to suggest B19 infection of nonerythroid lineage cells with antibody-dependent enhancement. (C) 2005 Elsevier Inc. All rights reserved.