Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.:: psychrophilic bacteria from Antarctic sea-ice habitats
Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.:: psychrophilic bacteria from Antarctic sea-ice habitats
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DOI：
10.1099/00207713-48-4-1213
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发表时间：
1998-10-01
期刊：
影响因子：
--
通讯作者：
McMeekin, TA
中科院分区：
文献类型：
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作者：
Bowman, JP;McCammon, SA;McMeekin, TA
从东南极塔斯马尼亚大学海冰岩芯中分离到的一组有色的、嗜冷的、严格有氧的化学异养菌，被发现代表了变形杆菌伽玛亚类中的一个新的165rRNA谱系，与Alteromonas属相邻。这些分离物是能动的、革兰氏阴性的杆状细胞，嗜冷和轻度嗜盐，对海水有绝对的需求。表型特征和DNA-DNA杂交结果表明，两个菌株形成了两个不同的类群，DNAG+C含量分别为44-46mol%和40mol%。然而，这些菌株的全细胞脂肪酸谱非常相似，主要脂肪酸成分为16：1 omega 7c、18：1 omega 7c、16：0和17：1 omega 8c。总体而言，存在着足够的差异来区分海冰菌株和目前公认的细菌属。海冰菌株代表了一个新属--Glaciecola，该属包含两个种：Glaciecola puicea gen.Nov.，sp.11月(ACAM611(T))和苍白冰盖(Glaciecola pallidula gen.)Nov.，sp.11月(ACAM615(T))。
A group of pigmented, psychrophilic, strictly aerobic chemoheterotrophs isolated from sea-ice cores collected from coastal areas of eastern Antarctica University of Tasmania, was found to represent a novel 165 rRNA lineage within the gamma subclass Of the Proteobacteria, adjacent to the genus Alteromonas. The isolates are motile, Gram-negative, rod-shaped cells, which are psychrophilic and slightly halophilic, and possess an absolute requirement for seawater. Differences in phenotypic characteristics and DNA-DNA hybridization indicated the isolates formed two distinct taxa which have DNA G+C contents of 44-46 mol % and 40 mol%, respectively. Whole-cell fatty acid profiles of the isolates were however very similar and included 16:1 omega 7c, 18:1 omega 7c, 16:0 and 17:1 omega 8c as the major fatty acid components. Overall, sufficient differences exist to distinguish the sea-ice strains from currently recognized bacterial genera. It is proposed the sea-ice strains represent a new genus, Glaciecola, which contains two species, Glaciecola punicea gen. nov., sp. nov. (ACAM 611(T)) and Glaciecola pallidula gen. nov., sp. nov. (ACAM 615(T)).