Ionic Mechanisms for Electrical Heterogeneity between Rabbit Purkinje Fiber and Ventricular Cells
Ionic Mechanisms for Electrical Heterogeneity between Rabbit Purkinje Fiber and Ventricular Cells
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DOI：
10.1016/j.bpj.2010.02.033
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发表时间：
2010-06-02
影响因子：
3.4
通讯作者：
Zhang, Henggui
中科院分区：
文献类型：
--
作者：
Aslanidi, Oleg V.;Sleiman, Rakan N.;Zhang, Henggui
浦肯野纤维（PF）与心室壁之间以及心室壁内部的电动作电位（AP）特性的内在不均匀性在确保心室成功兴奋方面发挥着重要作用。它也可能是由于浦肯野-心室交界处的不均匀复极所致。然而，离子机制的基础上，显着的AP之间的差异PF和心室细胞没有完全的特点。我们研究了这种机制，通过开发一个新的家庭的生物病理学详细的AP模型兔PF细胞和三个透壁心室细胞类型。该模型基于并验证了从兔离子通道，单细胞和组织水平上记录的实验数据。然后，他们被用来确定每个单独的离子通道电流在调节AP异质性在兔浦肯野-心室交界处的功能作用，并确定特定的电流负责的PF和心室细胞的药理干预的差异反应。
The intrinsic heterogeneity of electrical action potential (AP) properties between Purkinje fibers (PFs) and the ventricular wall, as well as within the wall, plays an important role in ensuring successful excitation of the ventricles. It can also be proarrhythmic due to nonuniform repolarization across the Purkinje-ventricular junction. However, the ionic mechanisms that underlie the marked AP differences between PFs and ventricular cells are not fully characterized. We studied such mechanisms by developing a new family of biophysically detailed AP models for rabbit PF cells and three transmural ventricular cell types. The models were based on and validated against experimental data recorded from rabbit at ionic channel, single cell, and tissue levels. They were then used to determine the functional roles of each individual ionic channel current in modulating the AP heterogeneity at the rabbit Purkinje-ventricular junction, and to identify specific currents responsible for the differential response of PFs and ventricular cells to pharmacological interventions.