Induction of mating in Candida albicans by construction of MTLa and MTLα strains
Induction of mating in Candida albicans by construction of MTLa and MTLα strains
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DOI：
10.1126/science.289.5477.310
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发表时间：
2000-07-14
期刊：
影响因子：
56.9
通讯作者：
Magee, PT
中科院分区：
文献类型：
--
作者：
Magee, BB;Magee, PT
虽然二倍体真菌白色念珠菌，一种人类病原体，一直被认为没有性周期。它通常具有酿酒酵母交配型基因（MAT）α和α的交配型样直系同源物（Mn）。当通过缺失第5号染色体的一个同源物构建仅含有MTLa或MTL α的菌株时，MTL基因座的位点、MTLa和MTL α菌株发生交配，但类似的交配类型却没有发生。交配的证据包括形成稳定的原养型菌株与互补营养缺陷型标记，这些包含MTL等位基因和分子标记，从父母和四倍体的DNA含量和单核。
Although the diploid fungus Candida albicans, a human pathogen, has been thought to have no sexual cycle. it normally possesses mating-type-like orthologs (Mn) of both of the Saccharomyces cerevisiae mating-type genes (MAT) a and alpha. When strains containing only MTLa or MTL alpha were constructed by the Loss of one homolog of chromosome 5, the site of the MTL Loci, MTLa and MTL alpha strains mated, but like mating types did not. Evidence for mating included formation of stable prototrophs from strains with complementing auxotrophic markers; these contained both MTL alleles and molecular markers from both parents and were tetraploid in DNA content and mononucleate.