Hepatitis E virus in pork production chain in Czech Republic, Italy, and Spain, 2010.
Hepatitis E virus in pork production chain in Czech Republic, Italy, and Spain, 2010.
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DOI：
10.3201/eid1808.111783
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发表时间：
2012-08
影响因子：
11.8
通讯作者：
Ruggeri FM
中科院分区：
文献类型：
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作者：
Di Bartolo I;Diez-Valcarce M;Vasickova P;Kralik P;Hernandez M;Angeloni G;Ostanello F;Bouwknegt M;Rodríguez-Lázaro D;Pavlik I;Ruggeri FM
处理不会显著减少内源性病毒。我们评估了2010年捷克共和国、意大利和西班牙猪肉生产链中戊型肝炎病毒（HEV）的流行情况。共337粪便，肝脏和肉类样品从屠宰场的动物进行了检测戊型肝炎病毒的实时定量PCR。总体而言，HEV在意大利（53%）和西班牙（39%）高于捷克共和国（7.5%）。在意大利（41%）和西班牙（39%）的粪便中以及捷克共和国的肝脏（5%）和肉类（2.5%）中最常检测到HEV。在加工和销售点取样的313根香肠中，仅在西班牙检测到戊型肝炎病毒（6%）。戊型肝炎病毒测序仅确认了g3戊型肝炎病毒株。指示病毒（猪腺病毒）在粪便样品中普遍存在，在肝脏样品中不存在，并在1份屠宰场肉样品中检测到。在销售时，我们在香肠中发现了猪腺病毒（1%-2%）。戊型肝炎病毒和其他粪便病毒可能通过猪肉生产传播，需要采取遏制措施。
Processing does not substantially abate endogenous virus. We evaluated the prevalence of hepatitis E virus (HEV) in the pork production chain in Czech Republic, Italy, and Spain during 2010. A total of 337 fecal, liver, and meat samples from animals at slaughterhouses were tested for HEV by real-time quantitative PCR. Overall, HEV was higher in Italy (53%) and Spain (39%) than in Czech Republic (7.5%). HEV was detected most frequently in feces in Italy (41%) and Spain (39%) and in liver (5%) and meat (2.5%) in Czech Republic. Of 313 sausages sampled at processing and point of sale, HEV was detected only in Spain (6%). HEV sequencing confirmed only g3 HEV strains. Indicator virus (porcine adenovirus) was ubiquitous in fecal samples and absent in liver samples and was detected in 1 slaughterhouse meat sample. At point of sale, we found porcine adenovirus in sausages (1%–2%). The possible dissemination of HEV and other fecal viruses through pork production demands containment measures.