PEPTIDE FRAGMENTATION SUITABLE FOR SOLID-PHASE MICROSEQUENCING - USE OF N-BROMOSUCCINIMIDE AND BNPS-SKATOLE [3-BROMO-3-METHYL-2-[(2-NITROPHENYL)THIO]-3H-INDOLE]
PEPTIDE FRAGMENTATION SUITABLE FOR SOLID-PHASE MICROSEQUENCING - USE OF N-BROMOSUCCINIMIDE AND BNPS-SKATOLE [3-BROMO-3-METHYL-2-[(2-NITROPHENYL)THIO]-3H-INDOLE]
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DOI：
10.1042/bj1870515
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发表时间：
1980-01-01
影响因子：
4.1
通讯作者：
WILSON, KJ
中科院分区：
文献类型：
--
作者：
HUNZIKER, PE;HUGHES, GJ;WILSON, KJ
使用BNPS-粪臭素[3-溴-3-甲基-2-[（2-硝基苯基）硫醇]-3H-吲哚]和N-溴琥珀酰亚胺在具有可用C-γ的氨基酸后的肽键处特异性裂解。dbd.C-.delta.双键即色氨酸、酪氨酸和组氨酸。所得的C-末端内酯方便地附着于氨基玻璃支持物上，用于用DABITC（4-N，N-二甲基氨基偶氮苯4“-异硫氰酸酯）测序。具有这种C-末端的肽，即，来自胰凝乳蛋白酶消化物，可以容易地与这些试剂反应，并且容易地通过固相方法连接和测序。
BNPS-skatole [3-bromo-3-methyl-2-[(2-nitrophenyl)thiol]-3H-indole] and N-bromosuccinimide were used to specifically cleave at peptide bonds after amino acids with available C-.gamma..dbd.C-.delta. double bonds. i.e. tryptophan, tyrosine and histidine. The resulting C-terminal lactones conveniently attach to amino-glass supports for sequencing with DABITC (4-NN-dimethylaminoazobenzene 4''-isothiocyanate). Peptides having such C-termini, i.e., from a chymotryptic digest, can be readily made to react with these reagents and be easily attached and sequenced by solid-phase methods.