Photosensitizer Effects of Artificial Red Cells on Dye Laser Irradiation in an Animal Model Assuming Port-Wine Stain Treatment
Photosensitizer Effects of Artificial Red Cells on Dye Laser Irradiation in an Animal Model Assuming Port-Wine Stain Treatment
复制标题
人工红细胞对葡萄酒色斑处理动物模型中染料激光照射的光敏效应
DOI：
--
复制
发表时间：
2017
影响因子：
3.6
通讯作者：
H. Sakai
中科院分区：
文献类型：
--
作者：
N. Rikihisa;Shoji Watanabe;K. Satoh;Yoshiaki Saito;H. Sakai
背景：葡萄酒污渍的完全去除仍然具有挑战性。基于染料激光治疗鲜红斑痣的原理，在激光治疗前立即静脉注射人造红细胞（血红蛋白囊泡）可能会改善治疗的临床结果。血红蛋白囊泡注射增加微血管中的血红蛋白浓度。染料激光的光子被红细胞和这种新添加的血红蛋白的混合物吸收，潜在地产生更多的热量和光凝固，并最终有效地使内皮细胞坏死。研究方法：为了确认血红蛋白囊泡作为光敏剂的性能，作者比较了血红蛋白囊泡和人血液对595 nm波长的吸光度以及染料激光照射后发生的温度升高。此外，作者还研究了静脉注射血红蛋白囊泡诱导的微血管转化。最后，作者研究了血红蛋白囊泡对染料激光照射鸡垂肉血管破坏的影响。结果：血红蛋白囊泡具有与人血相同的吸光度。它们在激光照射后产生与人体血液相同的热量。静脉注射血红蛋白囊泡可引起动物模型微血管扩张。在动物模型中，血红蛋白囊泡染料激光能有效地破坏血管壁。结论：血红蛋白囊泡可作为光敏剂破坏血管壁。脉冲染料激光抗性葡萄酒色斑的一个可能机制是过小的血管不包含足够的红细胞。因此，它们是这些激光器的不良吸收器/加热器。血红蛋白囊泡联合治疗将改善染料激光治疗仅针对此类病变的临床结果。
Background: The complete removal of port-wine stains has remained challenging. Based on the principle of treating port-wine stains with a dye laser, intravenous injection of artificial red cells (hemoglobin vesicles) immediately before laser treatment might improve the clinical outcome of the therapy. The hemoglobin vesicle injection increases the hemoglobin concentration in microvessels. Photons of dye laser are absorbed by the mixture of red blood cells and this newly added hemoglobin, potentially producing more heat and photocoagulation and, ultimately, necrosis of the endothelial cells effectively. Methods: To confirm the performance of hemoglobin vesicles as a photosensitizer, the authors compared the absorbance of hemoglobin vesicles and human blood against 595-nm wavelength and the temperature increases that occur following dye laser irradiation. Furthermore, the authors investigated the microvessel transformation induced by the hemoglobin vesicle intravenous injection. Finally, the authors investigated the effect of the hemoglobin vesicle on the vascular destruction of dye laser irradiation with chicken wattle. Results: Results show that hemoglobin vesicles have the same absorbance as that of human blood. They produce the same level of heat as human blood after laser irradiation. The hemoglobin vesicle intravenous injection caused dilatation of microvessels in animal models. The dye laser with hemoglobin vesicle can destroy the vessel wall effectively in animal models. Conclusions: Hemoglobin vesicles can function as photosensitizers to destroy the vessel wall. A possible mechanism of pulsed dye laser–resistant port-wine stains is that overly small vessels do not contain sufficient red blood cells. They are therefore poor absorbers/heaters for these lasers. Hemoglobin vesicle combination therapy will improve clinical outcomes of dye laser treatment against such lesions only.