Spatial and temporal changes of Decorin, Type I collagen and Fibronectin expression in normal and clone bovine placenta
Spatial and temporal changes of Decorin, Type I collagen and Fibronectin expression in normal and clone bovine placenta
复制标题
DOI：
10.1016/j.placenta.2014.06.366
复制
发表时间：
2014-09-01
期刊：
影响因子：
3.8
通讯作者：
Jammes, H.
中科院分区：
文献类型：
--
作者：
Guillomot, M.;Campion, E.;Jammes, H.
简介：在哺乳动物体细胞克隆后，包括细胞外基质成分在内的各种基因表达的改变在胎盘病理学中起主要作用。本研究的目的是分析的表达模式（mRNA和蛋白质）的小亮氨酸丰富的蛋白多糖，核心蛋白聚糖与I型胶原和纤维连接蛋白在牛胎盘组织从正常和克隆pregnances.Methods：核心蛋白聚糖的基因分型和等位基因表达的桑格测序确定。采用定量RT-qPCR、原位杂交和免疫组化相结合的方法，分析人工授精和体细胞克隆妊娠D18至足月子宫内膜和胎盘组织中核心蛋白聚糖、I型胶原和纤维连接蛋白1的表达模式。DCN在AI子宫内膜间质和绒毛膜间充质中的表达水平在着床过程中增加，在胎盘生长过程中下降直到学期结束联合ISH和IHC揭示了意想不到的差异mRNA和蛋白质组织分布。此外，核心蛋白聚糖维持在胎盘组织从SCNT妊娠，而mRNA和蛋白质都不存在于AI衍生的placenta.Discussion：在牛，核心蛋白聚糖的表达模式在胎盘形成过程中表现出显着的变化。核心蛋白聚糖的下调与胎盘组织的增殖、重塑和血管化有关。这些观察结果加强了假定的核心蛋白聚糖在这些processes.Conclusions的作用：这些观察结果表明，核心蛋白聚糖是参与胎盘的生长和失调，其表达与胎盘异常SCNT衍生妊娠。(C)2014爱思唯尔有限公司版权所有。
Introduction: Alteration of expression of various genes including extracellular matrix components, have been suggested to play major role in the placental pathologies after somatic cloning in mammals. The objectives of the present study were to analyze pattern of expression (mRNA and protein) of the small leucine-rich proteoglycan, Decorin in association with Type I Collagen and Fibronectin in bovine placental tissues from normal and clone pregnancies.Methods: Genotyping and allelic expression of Decorin were determined by Sanger sequencing. The expression patterns of Decorin, Type I collagen and Fibronectin 1 were analyzed by quantitative RT-qPCR and combined in situ hybydization (ISH) and immunohistochemistry (IHC) in endometrial and placental tissues from D18 to term from artificially inseminated and somatic cloning pregnancies.Results: The expression levels of DCN increased in the AI endometrial stroma and chorionic mesenchyme during implantation and declined during placentome growth until term. Combined ISH and IHC revealed an unexpected discrepancy mRNA and protein tissue distribution. Moreover, Decorin was maintained in the placentome tissues from SCNT pregnancies while both mRNA and protein were absent in AI derived placenta.Discussion: In bovine, the pattern of expression of Decorin exhibits significant changes during placental formation. Downregulation of Decorin is associated with proliferation, remodeling and vascularization of placental tissues. These observations reinforces the putative role of Decorin in these processes.Conclusions: These observations suggest that Decorin is involved in placental growth and that dysregulation of its expression is associated with placental abnormalities in SCNT derived pregnancy. (C) 2014 Elsevier Ltd. All rights reserved.