Quantification of staphylococcal-collagen binding interactions in whole blood by use of a confocal microscopy shear-adhesion assay.
Quantification of staphylococcal-collagen binding interactions in whole blood by use of a confocal microscopy shear-adhesion assay.
复制标题
使用共聚焦显微镜剪切粘附测定对全血中葡萄球菌-胶原蛋白结合相互作用进行定量。
DOI：
10.1086/375826
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Ross,JuliaM
中科院分区：
文献类型：
--
作者：
Mascari,LisaM;Ross,JuliaM
在血源性葡萄球菌感染中，细菌和血小板经常联合收割机，在暴露胶原的内皮下形成血栓。在这个过程中，胶原蛋白作为金黄色葡萄球菌的潜在结合表面。然而，S.金-胶原结合相互作用在感染血栓形成中的作用尚不确定。我们量化了S.在确定的生理相关流体剪切条件下，在全血悬浮液中，金黄色与胶原的粘附。金黄色葡萄球菌-胶原蛋白结合相互作用，由S.金黄色胶原粘附素（CNA）和蛋白A-血管性血友病因子（vWf），使用突变株和抗体阻断技术进行了评估。使用共聚焦激光显微镜测量粘附细菌的位置（在胶原蛋白表面处或在血小板聚集体中的表面上方）。结果表明在生理剪切条件下显著的CNA-胶原相互作用和蛋白A-vWf-胶原结合相互作用。我们的结论是，胶原蛋白结合的相互作用是重要的感染血栓的发展。
In bloodborne staphylococcal infections, bacteria and platelets often combine, forming thrombi on the subendothelium, where collagen is exposed. In this process, the collagen serves as a potential binding surface forStaphylococcus aureus. However, the extent and importance ofS. aureus-collagen binding interactions in the development of infected thrombi is uncertain. We quantifiedS. aureusadhesion to collagen in a whole-blood suspension under defined physiologically relevant fluid shear conditions.S. aureus-collagen binding interactions, mediated by both theS. aureuscollagen adhesin (CNA) and protein A-von Willebrand factor (vWf), were evaluated using mutant strains and antibody-blocking techniques. The position of adherent bacteria (at the collagen surface or above the surface in the platelet aggregate) was measured using confocal laser microscopy. Results demonstrated significant CNA-collagen interactions and protein A-vWf-collagen binding interactions under physiological shear conditions. We conclude that collagen binding interactions are important in the development of infected thrombi.