Effects of an isopropanolic-aqueous black cohosh extract on central body temperature of ovariectomized rats
Effects of an isopropanolic-aqueous black cohosh extract on central body temperature of ovariectomized rats
复制标题
异丙醇-黑升麻水提取物对去势大鼠中心体温的影响
DOI：
10.1016/j.jep.2011.08.076
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发表时间：
2011-10-31
影响因子：
5.4
通讯作者：
Zhou, Changman
中科院分区：
文献类型：
--
作者：
Ma, Xiaoyan;Zhang, Hui;Zhou, Changman
民族药理学相关性：黑升麻研究目的：本研究的目的是检查异丙醇黑升麻提取物（iCR）对卵巢切除大鼠（OVX）的中心体温（CBT）的影响，并阐述其在减轻更年期相关潮热中的可能作用。材料和方法：64只雌性SD大鼠，体重230 ± 10 g，6-8周龄，分为4组：卵巢切除（OVX）组、假手术组、卵巢切除加戊酸雌二醇（OVX + E）组和卵巢切除加iCR（OVX+ICR）组。假手术组进行假手术而不切除卵巢，而其他三组在无菌条件下进行双侧卵巢切除术，并将温度植入物嵌入所有四组的腹腔中。结果：4组动物的CBT均维持正常的昼夜节律，白天CBT低，夜间CBT高。假手术组CBTs低于其他三组。（OVX + E）组和（OVX + ICR）组的日CBTs分别从第2天和第22天开始低于OVX组。昼夜CBT差值以假手术组最小，OVX + E组和OVX + ICR组均高于OVX组。OVX组昼夜CBT波幅、5 min间隔CBT波动频率均高于Sham组，OVX + E组昼夜CBT波幅、（OVX + ICR）组的CBT值高于OVX组，（OVX+ICR）组的CBT值低于OVX组;结论：去卵巢大鼠体温调节异常，表现为昼夜CBT升高，昼夜CBT差值增大，昼夜CBT振幅增大，昼夜CBT波动频率增加。对于草药提取物iCR，影响异常体温调节的起效时间长于戊酸雌二醇。ICR对卵巢切除大鼠的白天CBT有显著影响，但对夜间CBT影响不大。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: Black cohosh (Cimicifuga racemosa) is widely used in menopause symptoms strategy.Aim of the study: The aim of this study was to examine the effect of isopropanolic black cohosh extract (iCR) on the central body temperature (CBT) of ovariectomized rats (OVX) and elaborate its possible effects in alleviating menopause related hot flushes.Materials and methods: 64 female Sprague-Dawley rats, weighing 230 +/- 10g and aged 6-8 weeks, were divided into four groups: ovariectomy (OVX), sham, ovariectomy plus estradiol valerate (OVX + E), and ovariectomy plus iCR (OVX+ICR). The sham group underwent a sham surgery without ovariectomies, while the other three groups underwent bilateral ovariectomies under sterile conditions and a temperature implant was embedded in the abdominal cavity of all four groups. After 2-week recovery period, the temperature of all animals was monitored for 6 weeks.Results: CBT of four groups maintained a normal circadian rhythm, with a low day CBT and a high night CBT. CBTs of the sham group were lower than that of the other three groups. The day CBTs of the (OVX + E) group and (OVX + ICR) group were lower than that of the OVX group from day 2 and day 22 respectively. For the difference between day and night CBT, the sham group was smallest, while (OVX + E) and (OVX + ICR) groups were higher than that of OVX group. The amplitude of day and night CBT, CBT fluctuation frequency at 5 min intervals, were higher for the OVX group than the sham group; the amplitude of day and night CBT of (OVX + E) group and the amplitude of night CBT of (OVX + ICR) group were higher than those of OVX group; while the amplitude of day CBT of (OVX+ICR) group was lower than that of OVX group; CBT fluctuation frequency at 5 min intervals was higher for the (OVX + E) and (OVX + ICR) groups than the OVX group.Conclusions: Ovariectomized rats had abnormal thermoregulation, demonstrating an increase in day and night CBT, greater difference between day and night CBT, higher amplitude of day and night CBT, and more CBT fluctuation frequency. For the herbal extract iCR, the onset of affecting abnormal thermoregulation took longer than that of estradiol valerate. ICR had a significant effect on day CBT but was only little effective on night CBT of ovariectomized rats. (C) 2011 Elsevier Ireland Ltd. All rights reserved.