Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy
Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy
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双模荧光显微内窥镜评估人宫颈癌细胞基因转染效果
DOI：
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发表时间：
2012
影响因子：
3.4
通讯作者：
Jin U. Kang
中科院分区：
文献类型：
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作者：
J. Cha;Jing Zhang;S. Gurbani;G. Cheon;Min Li;Jin U. Kang
我们报告了一种定量评估基因转染效果的新方法，使用双模态显微内窥镜，可以同时监测激光扫描反射和荧光成像。该系统采用直径为500 μm的相干光纤束，横向分辨率为3.5 μm。从两个硅雪崩光电探测器获得的反射和荧光图像在1hz下显示，并自动处理以计算基因转染效率（荧光细胞占总细胞的比例）。为了验证该系统的性能，我们使用人宫颈癌细胞（HeLa）在四种市售试剂中检测了青色荧光蛋白的表达。结果与高分辨率台式显微镜进行了比较。
We report a novel approach to quantitatively assess gene transfection efficacy using dual-modality microendoscopy that can simultaneously monitor both laser scanning reflectance and fluorescence imaging. The system uses a 500-μm-diameter coherent fiber bundle and permits 3.5-μm lateral resolution. Both reflectance and fluorescence images obtained from two silicon avalanche photodetectors are displaying at 1 Hz and processed automatically to calculate gene transfection efficiency (the ratio of fluorescent cells among the total cells). To validate the system performance we examined the expression of cyan fluorescent protein using human cervical cancer cells (HeLa) in four commercially available reagents. The result was compared with that using a high-resolution bench-top microscope.