Photogenotoxicity of Mammalian Cells: A Review of the Different Assays for In Vitro Testing¶
Photogenotoxicity of Mammalian Cells: A Review of the Different Assays for In Vitro Testing¶
复制标题
哺乳动物细胞的光基因毒性：体外测试不同测定方法的回顾¶
DOI：
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发表时间：
2002
影响因子：
3.3
通讯作者：
L. Marrot
中科院分区：
文献类型：
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作者：
J. Meunier;A. Sarasin;L. Marrot
摘要近年来，人们在分子水平上对光毒性进行了研究，这些研究为DNA损伤的表征、DNA修复和细胞对紫外线胁迫的反应提供了新的视角。新型抗生素和抗菌药物的开发突出了在新化合物的安全性评价中开展光毒性评价的必要性。本文综述了紫外线诱导的细胞应激反应的分子机制、体外筛选太阳光毒性的方法以及光激活药物的光敏化过程。列出了该特定目标中感兴趣的紫外线源、生物系统和终点。光毒性效应从细胞毒性-凋亡效应到诱导原发性DNA损伤、DNA修复和作用于细胞周期和细胞命运的各种应激基因。最终，它可以导致遗传DNA修饰的诱导。提出了各种测定法来专门解决紫外线诱导毒性的所有这些特定后果。
Abstract During the past several years, phototoxicity has been studied at the molecular level, and these studies have provided new insights in the field of DNA lesion characterization, DNA repair and cell response to ultraviolet (UV)-induced stress. The development of new antibiotics and antiinflammatory drugs has highlighted the necessity to develop the assessment of phototoxicity in the safety evaluation of new chemical compounds. This paper aims at reviewing the known molecular mechanisms of the cellular response to UV-induced stress, the in vitro methods that can be proposed and used to screen for toxicity of sunlight and the photosensitization process resulting from the activation of drugs by light. UV sources, biological systems and endpoints of interest in that particular objective are listed. Phototoxic effects span from the cytotoxic–apoptotic effect to the induction of primary DNA damage, DNA repair and a variety of stress genes acting on the cell cycle and the fate of the cell. Ultimately, it can lead to the induction of hereditary DNA modification. A variety of assays are proposed to specifically address all these particular consequences of UV-induced toxicity.
DOI：
10.1073/pnas.88.22.10124
发表时间：
1991-11-01
影响因子：
11.1
作者：
BRASH, DE;RUDOLPH, JA;PONTEN, J
通讯作者：
PONTEN, J