Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1
Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1
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DOI：
10.1094/mpmi-19-0123
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发表时间：
2006-02-01
影响因子：
3.5
通讯作者：
Shah, J
中科院分区：
文献类型：
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作者：
Makandar, R;Essig, JS;Shah, J
小麦枯萎病是小麦和大麦的一种毁灭性病害，在世界范围内造成了广泛的损失。单基因、基因对基因的FHB耐药尚未见报道。抗赤霉病的最佳来源是一个复杂的数量性状，来源于小麦变异。麦3号。本研究表明，调控系统性获得性抗性激活的拟南芥NPR1基因（AtNPR1）在fhb易感小麦cv中表达。山齿鹑，赋予一种遗传的，11型抵抗由镰刀菌引起的FHB。表达atnpr1的转基因小麦对赤霉病抗性的增强与受到赤霉病侵袭时更快的激活或防御反应有关。在表达atnpr1的小麦的真菌侵染穗中，PR1的表达被迅速诱导到高水平。此外，水杨酸的功能类似物苯并噻二唑在表达atnpr1的小麦中诱导PR1的表达速度和水平高于非转基因植株。我们认为，表达atnpr1的小麦对FHB的抗性是由于这些植物对内源性植物防御激活剂的反应更敏感。结果表明，NPR1是控制FHB的有效候选基因。
Fusarium bead blight (FHB) is a devastating disease of wheat and barley which causes extensive losses worldwide. Monogenic, gene-for-gene resistance to FHB has not been reported. The best source of resistance to FHB is a complex, quantitative trait derived from the wheat cv. Sumai 3. Here, we show that the Arabidopsis thaliana NPR1 gene (AtNPR1), which regulates the activation of systemic acquired resistance, when expressed in the FHB-susceptible wheat cv. Bobwhite, confers a heritable, type 11 resistance to FHB caused by Fusarium graminearum. The heightened FHB resistance in the transgenic AtNPR1-expressing wheat is associated with the faster activation or defense response when challenged by the fungus. PR1 expression is induced rapidly to a high level in the fungus-challenged spikes of the AtNPR1-expressing wheat. Furthermore, benzothiadiazole, a functional analog of salicylic acid, induced PR1 expression faster and to a higher level in the AtNPR1-expressing wheat than in the nontransgenic plants. We suggest that FHB resistance in the AtNPR1-expressing wheat is a result of these plants being more responsive to an endogenous activator of plant defense. Our results demonstrate that NPR1 is an effective candidate for controlling FHB.