MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis
MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis
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DOI：
10.1105/tpc.106.048017
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发表时间：
2007-07-01
期刊：
影响因子：
11.6
通讯作者：
Kazan, Kemal
中科院分区：
文献类型：
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作者：
Dombrecht, Bruno;Xue, Gang Ping;Kazan, Kemal
拟南芥碱性螺旋-环-螺旋Leu拉链转录因子（TF）MYC 2/JIN 1差异调节茉莉酸（JA）响应性病原体防御（例如，PDF1.2）和伤口反应（e.例如，在一个实施例中，VSP）基因。在这项研究中，野生型和突变myc 2/jin 1植物的全基因组转录谱功能分析揭示了MYC 2在不同JA功能调节中的新作用。我们发现MYC 2在JA信号传导过程中负调控Trp和Trp衍生的次级代谢，例如吲哚硫代葡萄糖苷的生物合成。此外，MYC 2正调控JA介导的对昆虫害虫（如棉铃虫）的抗性和对氧化胁迫的耐受性，这可能是通过增强抗坏血酸氧化还原循环和类黄酮生物合成实现的。MYC 2顺式结合元件和MYC 2调节基因在MYC 2调节的TF的一个子集的T-DNA插入系中的表达的分析表明，MYC 2可能通过在JA信号传导中具有激活或抑制作用的TF的中间谱的差异调节来调节JA反应。MYC 2也负调节其自身的表达，这可能是微调JA信号传导的机制之一。总的来说，这些结果为MYC 2的功能和JA信号通路的转录协调提供了新的见解。
The Arabidopsis thaliana basic helix-loop-helix Leu zipper transcription factor (TF) MYC2/JIN1 differentially regulates jasmonate (JA)-responsive pathogen defense(e.g., PDF1.2) and wound response ( e. g., VSP) genes. In this study, genome-wide transcriptional profiling of wild type and mutant myc2/jin1 plants followed by functional analyses has revealed new roles for MYC2 in the modulation of diverse JA functions. We found that MYC2 negatively regulates Trp and Trp- derived secondary metabolism such as indole glucosinolate biosynthesis during JA signaling. Furthermore, MYC2 positively regulates JA-mediated resistance to insect pests, such as Helicoverpa armigera, and tolerance to oxidative stress, possibly via enhanced ascorbate redox cycling and flavonoid biosynthesis. Analyses of MYC2 cis binding elements and expression of MYC2-regulated genes in T-DNA insertion lines of a subset of MYC2-regulated TFs suggested that MYC2 might modulate JA responses via differential regulation of an intermediate spectrum of TFs with activating or repressing roles in JA signaling. MYC2 also negatively regulates its own expression, and this may be one of the mechanisms used in fine-tuning JA signaling. Overall, these results provide new insights into the function of MYC2 and the transcriptional coordination of the JA signaling pathway.