Detection and quantification of conjunctival mucins.
Detection and quantification of conjunctival mucins.
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结膜粘蛋白的检测和定量。
DOI：
10.1007/978-1-4615-5359-5_35
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发表时间：
1998
影响因子：
--
通讯作者：
Jumblatt,MM
中科院分区：
文献类型：
--
作者：
Jumblatt,JE;Jumblatt,MM
粘蛋白是由粘膜组织的杯状和非杯状上皮细胞分泌的一类高相对分子质量的糖蛋白。对人类呼吸道、肠道和乳腺粘蛋白的分子研究已经确定了8种不同的粘蛋白(MUC1-MUC8)，它们以不同的组织特异性模式表达。1粘蛋白典型地包含不同的富含丝氨酸和苏氨酸的串联重复结构域，这些重复结构域高度O-糖基化。粘蛋白辅基蛋白重复区的O-糖基化始于将N-乙酰氨基半乳糖(GalNAc)加到丝氨酸和苏氨酸残基上。随后详细阐述了直链和支链糖侧链，得到了含有50-85%碳水化合物的极高分子量(>；106Da)糖蛋白。
Mucins are a family of high-molecular-weight glycoproteins secreted by the goblet and non-goblet epithelial cells of mucosal tissues. Molecular studies of human respiratory, intestinal, and mammary mucins have identified eight distinct mucins (MUC1-MUC8), which are expressed in distinct tissue-specific patterns.1Mucins characteristically contain distinct serine- and threonine-rich tandem repeat domains that are heavily O-glycosylated. O-glycosylation of the repeat region of mucin apoproteins begins with the addition ofN-acetylgalactosamine (GalNAc) to serine and threonine residues. Straight and branched sugar side chains are subsequently elaborated, resulting in extremely high-molecular-weight (>106Da) glycoproteins containing 50–85% carbohydrate by weight.2