The plasminogen receptor directs maintenance of spermatogonial stem cells by targeting BMI1
The plasminogen receptor directs maintenance of spermatogonial stem cells by targeting BMI1
复制标题
纤溶酶原受体通过靶向 BMI1 来指导精原干细胞的维持
DOI：
10.1007/s11033-022-07289-1
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发表时间：
2022-02-27
影响因子：
2.8
通讯作者：
Wu, Yibo
中科院分区：
文献类型：
--
作者：
Zhou, Hui;Shen, Cong;Wu, Yibo
精原干细胞（spermatogonialstemcells，SSCs）是一种独特的干细胞，参与了男性的整个生殖生活，并将遗传信息传递给后代。SSC的维护是复杂的，基本机制基本上不清楚。方法与结果PLGRKT定位于精原干细胞中，在体外培养的新生大鼠睾丸和精原干细胞中，PLGRKT表达下调可抑制细胞增殖，促进细胞凋亡。PLGRKT与B淋巴瘤Mo-MLV插入区1（BMI 1）相互作用，调节SSC中的氧化应激和p16/p19信号转导。
BackgroundSpermatogonial stem cells (SSCs) are unique stem cells that account for the whole reproductive life of males and transmit genetic information to offspring. SSC maintenance is intricate and the underlying mechanisms are largely unclear. Here, we report that SSC maintenance is driven by the plasminogen receptor (PLGRKT).Methods and resultsPLGRKT was located in SSCs, and knockdown of PLGRKT expression in cultured neonatal testis and SSCs impaired the proliferation and promoted the apoptosis of cells. PLGRKT interacted with B lymphoma Mo-MLV insertion region 1 (BMI1), and modulated oxidative stress and p16/p19 signaling in SSCs.ConclusionsWe demonstrated that reactive oxygen species (ROS) and p16/p19 signaling are involved in “PLGRKT–BMI1” co-regulation of SSC maintenance in mice.