ASCORBIC-ACID AND DEHYDROASCORBIC ACID MEASUREMENTS IN HUMAN PLASMA AND SERUM
ASCORBIC-ACID AND DEHYDROASCORBIC ACID MEASUREMENTS IN HUMAN PLASMA AND SERUM
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DOI：
10.1093/ajcn/54.4.712
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发表时间：
1991-10-01
影响因子：
7.1
通讯作者：
LEVINE, M
中科院分区：
文献类型：
--
作者：
DHARIWAL, KR;HARTZELL, WO;LEVINE, M
我们研究了人类循环中的抗坏血酸是否是蛋白质结合的或游离的，以及抗坏血酸是否以其还原形式单独作为抗坏血酸或以其还原和氧化形式分别作为抗坏血酸和脱氢抗坏血酸存在。抗坏血酸和脱氢抗坏血酸采用高效液相色谱-库仑电化学检测法测定，蛋白结合采用离心超滤法测定。抗坏血酸游离在血浆和血清中的正常，健康志愿者，10名男性和10名女性。抗坏血酸仅以其还原形式被检测到。然而，脱氢抗坏血酸可以出现在氧化条件下处理的样品中。因为循环中的抗坏血酸是游离的，并且仅作为还原维生素被检测到，所以抗坏血酸可以在没有中间体的情况下用于外周利用。脱氢抗坏血酸可能不存在于正常人的血浆和血清中，除非测定条件允许抗坏血酸氧化。
We investigated whether circulating ascorbic acid in humans is protein bound or free and whether ascorbic acid exists in its reduced form alone as ascorbic acid or in its reduced and oxidized forms as ascorbic acid and dehydroascorbic acid, respectively. Ascorbic acid and dehydroascorbic acid were determined by using HPLC with coulometric electrochemical detection, and protein binding was determined by centrifugal ultrafiltration. Ascorbic acid was free in plasma and serum of normal, healthy volunteers, 10 men and 10 women. Ascorbic acid was detectable only in its reduced form. However, dehydroascorbic acid could be made to appear in samples processed under oxidizing conditions. Because circulating ascorbic acid is free and is detected only as reduced vitamin, ascorbic acid may be available without intermediates for peripheral utilization. Dehydroascorbic acid may not be present in plasma and serum of normal humans unless assay conditions permit ascorbic acid oxidation.