Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine
Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine
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DOI：
10.1177/2041731419881528
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发表时间：
2019-10-01
影响因子：
8.2
通讯作者：
Izumi, Kenji
中科院分区：
文献类型：
--
作者：
Hoshikawa, Emi;Sato, Taisuke;Izumi, Kenji
基于图像的细胞/集落分析提供了有前途的解决方案，以弥补缺乏质量控制（QC）工具来无创监测培养细胞，这是再生医学的监管挑战。在这里，两个图像分析算法，光流和归一化的互相关，非侵入性地测量细胞/集落运动在人类初级口腔角质形成细胞筛选细胞的增殖能力在细胞培养的早期阶段的可行性进行了研究。我们将我们的软件应用于从96个连续的口腔角质形成细胞培养的延时相衬图像转换的电影。在分割生长的菌落之后，基于每种算法计算两个指数。评价集落各项指标与其增殖能力的相关性。该软件能够非侵入性地评估细胞/集落运动，并且每个指数反映了观察到的细胞动力学。发现细胞/集落运动和增殖能力之间存在正线性相关性，表明这两种算法都是QC的潜在工具。
Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC.