Similar roles for yeast Dbp2 and Arabidopsis RH20 DEAD-box RNA helicases to Ded1 helicase in tombusvirus plus-strand synthesis
Similar roles for yeast Dbp2 and Arabidopsis RH20 DEAD-box RNA helicases to Ded1 helicase in tombusvirus plus-strand synthesis
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DOI：
10.1016/j.virol.2012.06.030
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发表时间：
2012-10-25
期刊：
影响因子：
3.7
通讯作者：
Nagy, Peter D.
中科院分区：
文献类型：
--
作者：
Kovalev, Nikolay;Barajas, Daniel;Nagy, Peter D.
招募的宿主因子有助于正链RNA病毒的复制。酵母Dbp2 DEAD-box解旋酶是人类p68 DEAD-box解旋酶的同源物，它直接影响番茄丛矮病毒(TBSV)的复制。在基于酵母的无细胞TBSV复制实验中，我们证明了Dbp2与病毒负链RNA的3‘端结合，并通过病毒复制酶增强正链合成。Wt和一个ATPase缺陷的Dbp2突变体的体外数据表明，Dbp2解开了TBSV(-)RNA 3‘端的局部二级结构。我们还发现，Dbp2在酵母中补充了TBSV的复制缺陷，Ded1死盒螺旋酶是参与TBSV复制的另一个宿主因子，这表明Dbp2和Ded1解旋酶在TBSV复制中起着多余的作用。我们还证明了拟南芥中同源的AtRH20 DEAD-BOX解旋酶在体外和酵母中都能促进番茄病毒的复制。(C)2012 Elsevier Inc.保留所有权利。
Recruited host factors aid replication of plus-strand RNA viruses. In this paper, we show that Dbp2 DEAD-box helicase of yeast, which is a homolog of human p68 DEAD-box helicase, directly affects replication of Tomato bushy stunt virus (TBSV). We demonstrate that Dbp2 binds to the 3'-end of the viral minus-stranded RNA and enhances plus-strand synthesis by the viral replicase in a yeast-based cell-free TBSV replication assay. In vitro data with wt and an ATPase-deficient Dbp2 mutant indicate that Dbp2 unwinds local secondary structures at the 3'-end of the TBSV (-)RNA We also show that Dbp2 complements the replication deficiency of TBSV in yeast containing reduced amount of Ded1 DEAD-box helicase, another host factor involved in TBSV replication, suggesting that Dbp2 and Ded1 helicases play redundant roles in TBSV replication. We also show that the orthologous AtRH20 DEAD-box helicase from Arabidopsis can increase tombusvirus replication in vitro and in yeast. (C) 2012 Elsevier Inc. All rights reserved.