The Use of Intracerebral Microdialysis to Elucidate Environmentally Induced Neurotoxic Mechanisms.
The Use of Intracerebral Microdialysis to Elucidate Environmentally Induced Neurotoxic Mechanisms.
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使用脑内微透析来阐明环境诱发的神经毒性机制。
DOI：
10.1002/cptx.72
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Lasley,StephenM
中科院分区：
文献类型：
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作者：
Lasley,StephenM
微透析技术可以评估神经递质活性并监测组织细胞外液中的其他细胞实体。该方法广泛用于定量生物胺和氨基酸递质、肽、给药药物和其他分子以响应各种实验治疗。本文概述了脑内微透析方法在神经毒理学领域用于阐明环境因素作用的方式。该技术以多种创造性的方式用于解决涉及多种有毒物质的特定实验目标。通过适当考虑方法参数，研究人员还能够解决研究中的机械问题。这些研究包括在各种环境金属暴露方案后对细胞外液中的神经递质进行采样，以及血脑屏障渗透性的评估、活性氧的检测以及环境因素的毒动力学的描述。本次检查的目的不是审查研究结果本身，而是强调该方法中使用的各种方法以及已解决的实验问题。 © 2019 John Wiley & Sons, Inc. 版权所有
The technique of microdialysis permits the assessment of neurotransmitter activity and the monitoring of other cellular entities in tissue extracellular fluid. The method is widely used for quantifying biogenic amine and amino acid transmitters, peptides, administered drugs, and other molecules in response to various experimental treatments. This article provides an overview of the manner in which the methodology of intracerebral microdialysis is utilized in the field of neurotoxicology to elucidate the actions of environmental agents. The technique is employed in a variety of creative ways to address specific experimental goals involving myriad toxicants. With appropriate consideration of method parameters, investigators have also been able to address mechanistic issues in their studies. These investigations consist of sampling of neurotransmitters in extracellular fluid after various protocols of environmental metal exposure as well as assessments of blood–brain barrier permeability, the detection of reactive oxygen species, and description of the toxicodynamics of environmental agents. The purpose of this examination is not to review the investigational findings, per se, but to highlight the various approaches utilized with this methodology and the experimental questions that have been addressed. © 2019 by John Wiley & Sons, Inc.