The antibacterial mode of action of allitridi for its potential use as a therapeutic agent against Helicobacter pylori infection
The antibacterial mode of action of allitridi for its potential use as a therapeutic agent against Helicobacter pylori infection
复制标题
allitridi 的抗菌作用方式及其作为幽门螺杆菌感染治疗剂的潜在用途。
DOI：
10.1111/j.1574-6968.2009.01877.x
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发表时间：
2010-02-01
影响因子：
2.1
通讯作者：
Jia, Jihui
中科院分区：
文献类型：
--
作者：
Liu, Shuang;Sun, Yundong;Jia, Jihui
传统的根除幽门螺杆菌方法在临床治疗中往往失败。因此，迫切需要一种新的有效的治疗药物。大蒜素是一种专利大蒜衍生物，已成功用于治疗中国的系统性真菌和细菌感染。我们先前的研究表明，大蒜素对幽门螺杆菌的生长具有剂量依赖性的抑制作用。然而，大蒜素的抗菌作用机制仍不清楚。蛋白质组学分析研究大蒜新素诱导的整体蛋白质变化。共有21个蛋白质点被鉴定为差异表达。我们的结果表明，大蒜素在幽门螺杆菌中的抑菌机制可归因于其在能量代谢和生物合成方面的多靶点抑制作用，包括氨基酸生物合成、蛋白质合成、mRNA合成和脂肪酸生物合成。大蒜素还可以干扰抗氧化蛋白的表达，减少毒力因子的产生。Western印迹分析表明，亚抑制浓度的大蒜素能有效抑制CagA和VacA的产生。我们对大蒜素的抗菌作用模式的研究为大蒜素作为抗幽门螺杆菌感染的治疗药物的潜在用途提供了洞察力。
Eradication of Helicobacter pylori with traditional therapy often fails in clinical treatment. As a result, a novel efficacious therapeutic agent is strongly needed. Allitridi, a proprietary garlic derivative, has been successfully used to treat both systemic fungal and bacterial infections in China. Our previous study has shown a dose-dependent inhibitory effect of allitridi on H. pylori growth. However, the antibacterial mode of action of allitridi is still unclear. Proteomic analysis was used to study the global protein alterations induced by allitridi. A total of 21 protein spots were identified to be differentially expressed. Our results indicated that the bacteriostatic mechanism of allitridi in H. pylori can be attributed to its multitarget inhibitory effects in energy metabolism and biosynthesis including amino acid biosynthesis, protein synthesis, mRNA synthesis and fatty acid biosynthesis. Allitridi can also disturb the expression of antioxidant proteins and decrease the production of virulence factors. Western blot analysis showed that allitridi at subinhibitory concentrations can potently suppress the production of CagA and VacA. Our investigations on the antibacterial mode of action of allitridi provide an insight into the potential use of allitridi as a therapeutic agent against H. pylori infection.