High juvenile hormone titer and abdominal activation of the JH signaling may induce reproduction of termite neotenics.
High juvenile hormone titer and abdominal activation of the JH signaling may induce reproduction of termite neotenics.
复制标题
高保幼激素滴度和腹部 JH 信号激活可能诱导白蚁幼态繁殖。
DOI：
10.1111/imb.12169
复制
发表时间：
2015
影响因子：
2.6
通讯作者：
Maekawa K
中科院分区：
文献类型：
--
作者：
Saiki R;Gotoh H;Toga K;Miura T;Maekawa K
白蚁的种姓是一个重要的例子，其中生殖分工是明确的殖民地。白蚁的生殖等级包括初级生殖等级和幼态生殖等级;初级生殖等级发现一个新的群体，而幼态生殖等级在初级生殖等级死亡或衰老时接替它们的生殖角色。幼态分化通常通过发育功能性性腺同时保留幼态特征而与雌、雄工蚁分化;然而，幼态分化过程中的发育机制仍然知之甚少。保幼激素（JH）介导了白蚁种姓分化的许多方面的发育调控。在本研究中，我们定量的JH滴度在幼态生殖的散白蚁speratus，并与其他发育阶段进行比较。此外，在幼态分化期间，研究了头部、胸部和腹部中JH信号传导基因同源物（耐甲氧普烯[Met]、Krüppel‐ homolog 1、Broad‐Complex）的表达变化。最后，我们通过RNA干扰（RNAi）研究了Metin在幼态生殖中的作用。结果表明，幼老期个体的JH滴度显著高于青壮年和工人。JH信号基因在幼态幼体中的表达高于工蜂和蛹，MetRNAi抑制了卵黄发生基因在新蜕皮幼态幼体中的表达。这些结果表明，幼态生育力可能是由JH滴度的大幅增加和JH信号通路的身体部位特异性激活控制的。
Termite castes are a key example of polyphenism, in which reproductive division of labour is clearly seen in colonies. The reproductive castes in termites include primary and neotenic reproductives; primary reproductives found a new colony whereas neotenics succeed them in the reproductive role when the primary reproductives die or become senescent. Neotenics usually differentiate from nymphs or workers by developing functional gonads while retaining juvenile characteristics; however, the developmental mechanism during neotenic differentiation remains poorly understood. Juvenile hormone (JH) mediates a number of aspects of developmental regulation in caste differentiation in termites. In the present study we quantified JH titres in neotenic reproductives ofReticulitermes speratus, and compared these with other developmental stages. In addition, expression changes in JH signalling gene homologues (Methoprene‐tolerant[Met],Krüppel‐homolog1, Broad‐Complex) in the head, thorax and abdomen were investigated during neotenic differentiation. Finally, we examined the function ofMetin reproduction of neotenics by RNA interference (RNAi). Our results showed that the JH titres of neotenics were significantly higher than those of nymphs and workers. JH signalling genes were highly expressed in neotenic abdomens, compared with those in workers and nymphs.MetRNAi resulted in the inhibition ofvitellogeningene expression in newly moulted neotenics. These results suggest that the fertility of neotenics might be controlled by a large increase of JH titres and body‐part‐specific activation of JH signalling pathways.