Eph receptor and ephrin signaling in developing and adult brain of the honeybee (Apis mellifera).
Eph receptor and ephrin signaling in developing and adult brain of the honeybee (Apis mellifera).
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蜜蜂 (Apis mellifera) 发育中和成年大脑中的 Eph 受体和肝配蛋白信号传导。
DOI：
10.1002/dneu.20341
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发表时间：
2007
影响因子：
3
通讯作者：
Maleszka,Ryszard
中科院分区：
文献类型：
--
作者：
Vidovic,Maria;Nighorn,Alan;Koblar,Simon;Maleszka,Ryszard
Eph受体酪氨酸激酶和ephrin信号传导在脊椎动物脑发育中的作用已被充分确立。它们参与哺乳动物突触结构和生理学的调节也正在出现。然而，很少有人知道它们对大脑发育的影响及其在成年无脊椎动物神经系统中的功能。在这里，我们报告的表征Eph受体和ephrin的直系同源物在蜜蜂，意大利蜜蜂（Am），和他们的作用在学习和memory.In原位杂交mRNA表达的表达表明，这两个基因的表达在整个发展蛹和成人大脑的均匀分布。然而，用Fc融合蛋白原位标记表明AmEphR和Amephrin蛋白差异定位于蘑菇体的细胞体区域以及触角和视叶的发育中的神经堆。在成年人中，AmEphR蛋白定位于视叶，触角叶的肾小球和蘑菇体的内侧叶中的突触接触区域。后两个区域与蜜蜂的嗅觉学习和记忆有关。将EphR-Fc和ephrin-Fc蛋白注射到成年蜜蜂的大脑中，在鼻子延伸反射的嗅觉条件反射之前1小时，24小时后显著降低记忆。注射ephrin-Fc的组中的实验性遗忘在训练后1小时明显。训练后注射ephrin-Fc也诱导了实验性遗忘，表明其在回忆中的作用。这是首次证明Eph分子可以调节昆虫记忆的形成。© 2006 Wiley Periodicals，Inc. Develop Neurobiol 67：233-251，2007.
Roles for Eph receptor tyrosine kinase and ephrin signaling in vertebrate brain development are well established. Their involvement in the modulation of mammalian synaptic structure and physiology is also emerging. However, less is known of their effects on brain development and their function in adult invertebrate nervous systems. Here, we report on the characterization of Eph receptor and ephrin orthologs in the honeybee,Apis mellifera(Am), and their role in learning and memory.In situhybridization for mRNA expression showed a uniform distribution of expression of both genes across the developing pupal and adult brain. However,in situlabeling with Fc fusion proteins indicated that theAmEphR andAmephrin proteins were differentially localized to cell body regions in the mushroom bodies and the developing neuropiles of the antennal and optic lobes. In adults,AmEphR protein was localized to regions of synaptic contacts in optic lobes, in the glomeruli of antennal lobes, and in the medial lobe of the mushroom body. The latter two regions are involved in olfactory learning and memory in the honeybee. Injections of EphR‐Fc and ephrin‐Fc proteins into the brains of adult bees, 1 h before olfactory conditioning of the proboscis extension reflex, significantly reduced memory 24 h later. Experimental amnesia in the group injected with ephrin‐Fc was apparent 1 h post‐training. Experimental amnesia was also induced by post‐training injections with ephrin‐Fc suggesting a role in recall. This is the first demonstration that Eph molecules function to regulate the formation of memory in insects. © 2006 Wiley Periodicals, Inc. Develop Neurobiol 67: 233–251, 2007.