Phenotypic and Molecular Identification of Sporothrix Isolates from an Epidemic Area of Sporotrichosis in Brazil
Phenotypic and Molecular Identification of Sporothrix Isolates from an Epidemic Area of Sporotrichosis in Brazil
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DOI：
10.1007/s11046-011-9437-3
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发表时间：
2011-10-01
期刊：
影响因子：
5.5
通讯作者：
Zancope-Oliveira, Rosely Maria
中科院分区：
文献类型：
--
作者：
Evangelista Oliveira, Manoel Marques;Almeida-Paes, Rodrigo;Zancope-Oliveira, Rosely Maria
在过去十年中，巴西的孢子虫病显著增加，特别是在里约热内卢州，发生了与猫向人传播的人畜共患病有关的流行病。最近，四个新的系统发育物种被纳入孢子丝菌物种复合体的基础上的表型和分子特征，并提出了一个新的种名（Sporothrix brasiliensis）的一些孢子丝菌菌株从这次流行。本研究描述了1998年至2008年期间从感染性皮肤病实验室（IPEC-FIOCRUZ）的患者中获得的246株分离株以及一份环境样本的特征。206株（83.4%）为S. brasiliensis，S. schenckii和S.墨西哥。25株（10.1%）不能根据其表型进行鉴定，并被归类为孢子丝菌属。对钙调素基因进行测序以确认这些分离株的身份。分子生物学鉴定结果表明，其中24株为S. brasiliensis，其余为S.球形藻分离物该分离物的表型特征为S. mexicana在S.申克氏分支本研究中描述的分子数据和表型特征之间的相关性是鉴定孢子丝菌复合体的基础。
Sporotrichosis has significantly increased in Brazil in the last decade, particularly in the state of Rio de Janeiro, with the occurrence of an epidemic related to zoonotic transmission from cats to humans. Recently, four new phylogenetic species were incorporated into the Sporothrix species complex based on the phenotypic and molecular characteristics, and a new species name (Sporothrix brasiliensis) was proposed for some of the Sporothrix isolates from this epidemic. This study describes the characterization of 246 isolates obtained from patients attending the Laboratory of Infectious Dermatology, IPEC-FIOCRUZ, between 1998 and 2008, together with one environmental sample. Two hundred and six of the isolates (83.4%) were characterized as S. brasiliensis, 15 (6.0%) as S. schenckii, and one (0.5%) as S. mexicana. Twenty-five isolates (10.1%) could not be identified according to their phenotype and were classified as Sporothrix spp. The calmodulin gene was sequenced to confirm the identity of these isolates. The molecular analysis demonstrated that 24 of the isolates were S. brasiliensis, with the remainder being a S. globosa isolate. The isolate characterized phenotypically as S. mexicana was clustered on the S. schenckii clade. The correlation between molecular data and phenotypic characteristics described in this study is fundamental to the identification of the Sporothrix complex.