Prevalence, antimicrobial resistance and genetic lineages of Enterococcus spp. from vegetable food, soil and irrigation water in farm environments in Tunisia
Prevalence, antimicrobial resistance and genetic lineages of Enterococcus spp. from vegetable food, soil and irrigation water in farm environments in Tunisia
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DOI：
10.1002/jsfa.7264
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发表时间：
2016-03-30
影响因子：
4.1
通讯作者：
Torres, Carmen
中科院分区：
文献类型：
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作者：
Ben Said, Leila;Klibi, Naouel;Torres, Carmen
背景：本研究的目的是确定不同环境下肠球菌的种类、克隆多样性、抗生素耐药性和毒力。将 71 个农场来源样品（34 个食用蔬菜、27 个土壤和 10 个灌溉水）和来自 5 个市场的 19 个蔬菜样品接种到添加或不添加庆大霉素的 Slanetz-Bartley 琼脂平板（分别为 SB-Gen 和 SB 平板）中进行肠球菌回收。 结果：SB 培养基中 72.2% 的测试样品获得肠球菌（农场食用蔬菜 88.2%；来自农场的食用蔬菜 88.2%；土壤和灌溉水，51%；市场食用蔬菜，84.2%），并获得 65 株肠球菌分离株。屎肠球菌是最常见的物种（52.3%），其次是希拉肠球菌（35.4%）、粪肠球菌（6.15%）和卡氏肠球菌（6.15%）。这些肠球菌中检测到的抗生素耐药性如下（百分比/检测基因）：环丙沙星（60%）、红霉素（18.4%/erm(B)）、四环素（15.4%/tet(M)-tet(L)）、卡那霉素（15.4%/aph(3')-III）、氯霉素（7.7%）、链霉素(3%/ant(6))、万古霉素 (6.15%/vanC2))、替考拉宁 (0%) 和氨苄西林 (0%)。在 90 个测试样本中，有 14 个 (15.5%) 在 SB-Gen 平板上检测到高水平庆大霉素耐药 (HLR-G) 肠球菌，并鉴定了 15 个分离株：10 个粪肠球菌、4 个粪肠球菌和 1 个海拉肠球菌。所有 HLR-G 肠球菌均携带 aac(6')-aph(2'')、erm(B) 和 tet(M) 基因以及其他抗性基因。 HLR-G 分离株表现出高度的遗传多样性（十种不同的 PFGE 图谱），并归因于序列类型 ST2、ST16、ST28 和新 ST528（粪肠球菌），以及 ST56、新 ST885 和新 ST886（粪肠球菌）。 结论：农场或市场环境中的食用蔬菜经常受到 HLR-G 肠球菌的污染，这些微生物可以通过如果不遵守卫生条件，就会影响食物链。 (C) 2015年化学工业学会
BACKGROUND: The objective of this study was to determine the species, clonal diversity, antibiotic resistance and virulence of enterococci in different environments. Seventy-one samples of farm origin (34 of food vegetables, 27 of soil and ten of irrigation water) and 19 samples of vegetables from five markets, were inoculated in Slanetz-Bartley agar plates supplemented or not with gentamicin (SB-Gen and SB plates, respectively) for enterococci recovery.RESULTS: Enterococci were obtained from 72.2% of tested samples in SB media (food vegetables from farms, 88.2%; soil and irrigation water, 51%; food vegetables from markets, 84.2%), and 65 enterococcal isolates were obtained. Enterococcus faecium was the most prevalent species (52.3%), followed by E. hirae (35.4%), E. faecalis (6.15%), and E. casseliflavus (6.15%). Antibiotic resistance detected among these enterococci was as follows (percentage/detected gene): ciprofloxacin (60%), erythromycin (18.4%/ erm(B)), tetracycline (15.4%/tet(M)-tet(L)), kanamycin (15.4%/aph(3')-III), chloramphenicol (7.7%), streptomycin (3%/ant(6)), vancomycin (6.15%/vanC2)), teicoplanin (0%) and ampicillin (0%). High-level gentamicin-resistant (HLR-G) enterococci were detected in SB-Gen plates in 14 of 90 tested samples (15.5%), and 15 isolates were characterized: ten E. faecalis, four E. faecium and one E. hirae. All HLR-G enterococci carried the aac(6')-aph(2 ''), erm(B) and tet(M) genes, among other resistance genes. The HLR-G isolates showed high genetic diversity (ten different PFGE profiles), and were ascribed to the sequence types ST2, ST16, ST28 and new ST528 (in E. faecalis), and ST56, new ST885 and new ST886 (in E. faecium).CONCLUSION: Food vegetables in the farm or market settings are frequently contaminated by HLR-G enterococci, and these microorganisms could reach the human intestine through the food chain, if hygienic conditions are not followed. (C) 2015 Society of Chemical Industry