Co-exposure of carboxyl-functionalized single-walled carbon nanotubes and 17α-ethinylestradiol in cultured cells: effects on bioactivity and cytotoxicity.
Co-exposure of carboxyl-functionalized single-walled carbon nanotubes and 17α-ethinylestradiol in cultured cells: effects on bioactivity and cytotoxicity.
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DOI：
10.1021/es504216a
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发表时间：
2014-11
影响因子：
11.4
通讯作者：
Maoyong Song;Fengbang Wang;Luzhe Zeng;Junfa Yin;Hailin Wang;G. Jiang
中科院分区：
文献类型：
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作者：
Maoyong Song;Fengbang Wang;Luzhe Zeng;Junfa Yin;Hailin Wang;G. Jiang
17-乙烯基雌二醇(17-α-lestediol，E_2)是环境雌激素的代表。虽然EE2可以与一些工程纳米颗粒(NPs)相互作用，但对NP相关的EE2在生物体中的生物活性知之甚少。在这项研究中，我们研究了共暴露的羧基功能化单壁碳纳米管(cf-SWCNTs)与EE2在人乳腺腺癌细胞株(MCF-7细胞)中的联合作用，重点研究了其细胞毒性和生物活性。在有或没有吸附EE2的cf-SWCNTs作用下，线粒体活性、膜损伤和细胞凋亡没有显著差异。然而，吸附在cf-SWCNTs上的EE2的生物活性明显受到抑制。计算细胞内EE2的有效浓度表明，只有不到0.2%的总吸附EE2被释放，这表明在细胞暴露过程中，大部分EE2被保留在cf-SWCNTs上。此外，在含有5-20%胎牛血清的培养液中，即使孵育10天，吸附的EE2的生物活性也没有明显变化，表明吸附在cf-SWCNTs上的EE2在细胞培养液中是高度稳定的。这些结果标志着EE2很有可能在与环境相关的环境中被cf-SWCNTs吸附，从而影响其毒性和生物命运。这对今后的研究也很有吸引力，这些研究涉及核燃料与环境中污染物之间的联系的风险评估方法。
17α-Ethinylestradiol (EE2) is the representative of environmental estrogens. Although EE2 can interact with some engineered nanoparticles (NPs), little is known about the bioactivity of NP-associated EE2 in organisms. In this study, we investigated the combined effects of the co-exposed carboxyl-functionalized single-walled carbon nanotubes (cf-SWCNTs) and EE2 in the human breast adenocarcinoma cell line (MCF-7 cells), focusing on the cytotoxicity and bioactivity. There were no significant differences in mitochondrial activity, membrane damage, and cell apoptosis when exposed to cf-SWCNTs with and without adsorbed EE2. However, the bioactivity of adsorbed EE2 on cf-SWCNTs was significantly inhibited. The calculated effective concentration of EE2 in cultured cells showed that less than 0.2% of the total adsorbed EE2 was released, indicating that most EE2 was retained on the cf-SWCNTs during cellular exposure. Furthermore, there were no obvious changes in the bioactivity of adsorbed EE2 in the culture medium containing 5-20% fetal bovine serum (FBS), even up to 10 days of incubation, indicating that the adsorbed EE2 on cf-SWCNTs is highly stable in the cell culture medium. These results mark a promising possibility for EE2 to be adsorbed by cf-SWCNTs in environmentally relevant settings and thereby influenced its toxicity and biological fate. This is also tempting for future studies involving risk assessment ways for association between NPs and contaminants in the environment.