A new locus in Cytophaga hutchinsonii involved in colony spreading on agar surfaces and individual cell gliding.
A new locus in Cytophaga hutchinsonii involved in colony spreading on agar surfaces and individual cell gliding.
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DOI：
10.1093/femsle/fnv095
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发表时间：
2015-07
影响因子：
2.1
通讯作者：
Zhe Li;Cong Zhang;Sen Wang;Jing Cao;Weican Zhang;Xuemei Lu
中科院分区：
文献类型：
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作者：
Zhe Li;Cong Zhang;Sen Wang;Jing Cao;Weican Zhang;Xuemei Lu
Cytophaga hutchinsonii 通过一种未知的机制在表面上快速滑动，没有鞭毛和 IV 型菌毛，并且它可以通过一种新的机制有效地降解结晶纤维素。 Tn4351 转座子诱变用于鉴定新基因 CHU_1798，该基因对于琼脂表面上的菌落扩散至关重要。进一步的研究表明，CHU_1798 的破坏导致软琼脂和硬琼脂表面上的菌落不扩散，并且单个细胞在玻璃表面上滑动部分缺陷。只要细胞与纤维素直接接触，CHU_1798突变体就可以消化纤维素，但它不能降解埋在琼脂平板中的纤维素粉末。扫描电镜显示，在培养初期，单个突变细胞在纤维素纤维表面呈不规则排列，但当细胞较多时，呈规则的平行排列，并能沿纤维素纤维扩散。这些结果表明 CHU_1798 在 C. hutchinsonii 的运动中发挥着重要作用，并提供了对细胞运动与纤维素降解之间关系的深入了解。
Cytophaga hutchinsonii glides rapidly over surfaces by an unknown mechanism without flagella and type IV pili and it can degrade crystalline cellulose efficiently by a novel mechanism. Tn4351 transposon mutagenesis was used to identify a new gene, CHU_1798, essential for colony spreading on agar surfaces. Further study showed that disruption of CHU_1798 caused non-spreading colonies on both soft and hard agar surfaces and individual cells were partially deficient in gliding on glass surfaces. The CHU_1798 mutant could digest cellulose as long as the cells were in direct contact with the cellulose, but it could not degrade cellulose powder buried in the agar plate. Scanning electron microscopy showed that individual mutant cells arranged irregularly on the cellulose fiber surface at an early stage of incubation, but later showed a regular parallel arrangement when there were plenty of cells and could spread along the cellulose fibers. These results suggest that CHU_1798 plays an important role in the motility of C. hutchinsonii and provide insight into the relation between cell motility and cellulose degradation.