Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
复制标题
粪肠球菌70S核糖体的冷冻电子显微镜结构。
DOI：
10.1038/s41598-020-73199-6
复制
发表时间：
2020-10-01
影响因子：
4.6
通讯作者：
Jogl G
中科院分区：
文献类型：
--
作者：
Murphy EL;Singh KV;Avila B;Kleffmann T;Gregory ST;Murray BE;Krause KL;Khayat R;Jogl G
粪肠球菌（Enterococcus faecalis）是一种革兰氏阳性微生物，可引起人类严重感染，但与许多细菌病原体一样，耐药性已使许多常用抗生素无效。在这里，我们报告的冷冻电镜结构的E。faecalis 70S核糖体的全局分辨率为2.8 μ m。与大肠杆菌相比，结构差异集中在外周和溶剂暴露区域，而功能中心，包括抗生素结合位点，与其他细菌核糖体相似。三维分类后得到的五个类之间的亚基间构象的比较确定了几个旋转状态。大的核糖体亚基蛋白bL31，形成intersubunit桥梁的小核糖体亚基，假设不同的构象在五个类，揭示如何接触的小亚基保持整个intersubunit旋转。在五类之一中观察到的tRNA位于旋转核糖体状态下的嵌合pe/E位置。E. faecalis现在扩展了我们对细菌核糖体结构的认识，并可作为开发有效对抗该病原体的新型抗生素化合物的基础。
Enterococcus faecalis is a gram-positive organism responsible for serious infections in humans, but as with many bacterial pathogens, resistance has rendered a number of commonly used antibiotics ineffective. Here, we report the cryo-EM structure of the E. faecalis 70S ribosome to a global resolution of 2.8 Å. Structural differences are clustered in peripheral and solvent exposed regions when compared with Escherichia coli, whereas functional centres, including antibiotic binding sites, are similar to other bacterial ribosomes. Comparison of intersubunit conformations among five classes obtained after three-dimensional classification identifies several rotated states. Large ribosomal subunit protein bL31, which forms intersubunit bridges to the small ribosomal subunit, assumes different conformations in the five classes, revealing how contacts to the small subunit are maintained throughout intersubunit rotation. A tRNA observed in one of the five classes is positioned in a chimeric pe/E position in a rotated ribosomal state. The 70S ribosome structure of E. faecalis now extends our knowledge of bacterial ribosome structures and may serve as a basis for the development of novel antibiotic compounds effective against this pathogen.