Molecular characterization of clinical IMP-producing Klebsiella pneumoniae isolates from a Chinese Tertiary Hospital.
Molecular characterization of clinical IMP-producing Klebsiella pneumoniae isolates from a Chinese Tertiary Hospital.
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DOI：
10.1186/s12941-017-0218-9
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发表时间：
2017-06-19
影响因子：
5.7
通讯作者：
Yang J
中科院分区：
文献类型：
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作者：
Lai K;Ma Y;Guo L;An J;Ye L;Yang J
产IMP肺炎克雷伯菌(IMPKpn)在中国地区呈零星流行。与IMPKpn传播有关的机制尚不清楚。肺炎克雷伯菌对碳青霉烯类非敏感菌株来源于本院。分别用脉冲场凝胶电泳法(PFGE)、VITEK 2 AST试剂盒和多位点序列分型法(MLST)分析其遗传相关性、药物敏感性和序列类型(ST)。采用S1-PFGE、Southern印迹分析和多重聚合酶链式反应(多重PCR)方法进行质粒图谱分析。在2009年10月至2016年6月期间，共鉴定出25株非重复的IMPKpn分离株。PFGE结果表明，这些菌株属于20株遗传无关的IMPKpn菌株。MLST鉴定出了不同的STS。大多数菌株携带bla IMP-4，其次是bla imp-1。共鉴定出4种不相容类型，分别为A/C(n=2)、B/O(n=2)、L/M(n=1)和N(n=14)，另1种类型未能确定。IMPKpn分离株在本院呈零星流行。不同大小的INCN型质粒已成为介导BLA IMP基因在本院传播的主要平台。
IMP-producing Klebsiella pneumoniae (IMPKpn) exhibits sporadic prevalence in China. The mechanisms related to the spread of IMPKpn remain unclear. Carbapenem non-susceptible K. pneumoniae isolates were collected from our hospital. The genetic relatedness, antimicrobial susceptibility, as well as sequence types (ST) were analyzed by pulsed-field gel electrophoresis (PFGE), VITEK 2 AST test Kit, and multilocus sequence typing (MLST), respectively. S1-PFGE, Southern blot analysis and multiple PCR amplification were used for plasmid profiling. Between October 2009 and June 2016, 25 non-repetitive IMPKpn isolates were identified. PFGE results showed that these isolates belonged to 20 genetically unrelated IMPKpn strains. Diverse STs were identified by MLST. Most strains carried bla IMP-4, followed by bla IMP-1. Four incompatibility types of bla IMP-carrying plasmids were identified, which included A/C (n = 2), B/O (n = 2), L/M (n = 1) and N (n = 14), while type of other one plasmid failed to be determined. The IMPKpn isolates exhibited sporadic prevalence in our hospital. IncN types of plasmids with various sizes have emerged as the main platform mediating the spread of the bla IMP genes in our hospital.