Noninvasive visualization of in vivo release and intratumoral distribution of surrogate MR contrast agent using the dual MR contrast technique.
Noninvasive visualization of in vivo release and intratumoral distribution of surrogate MR contrast agent using the dual MR contrast technique.
复制标题
使用双 MR 对比技术对替代 MR 对比剂的体内释放和肿瘤内分布进行无创可视化。
DOI：
10.1016/j.biomaterials.2010.06.008
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发表时间：
2010
期刊：
影响因子：
14
通讯作者：
Kato,Yoshinori
中科院分区：
文献类型：
--
作者：
Onuki,Yoshinori;Jacobs,Igor;Artemov,Dmitri;Kato,Yoshinori
直接评价药物从载体的体内释放曲线是药物递送系统的临床需求，因为体外表征的药物释放与体内释放的相关性很差。本研究的目的是证明在体内的适用性的双重MR造影技术作为一个有用的工具，用于无创监测的稳定性和释放的药物载体，通过可视化在体内释放的封装替代MR造影剂从载体和其随后的瘤内分布的配置文件。该技术的重要方面是它将阳性和阴性造影剂合并在单个载体内。GdDTPA，超顺磁性氧化铁纳米粒子，5-氟尿嘧啶封装在纳米和微球组成的聚（d，l-丙交酯-共-乙交酯），这是用来作为一个模型载体。用人乳腺癌原位异种移植物进行体内研究。在这里展示的基于MR的技术已经能够可视化载体的递送，以及封装的阳性造影剂的释放和肿瘤内分布。这项研究证明了在体内释放和分布的MR造影剂的非侵入性监测的原理证明的结果，因此，这项技术将作出很大的贡献，该领域。
A direct evaluation of the in vivo release profile of drugs from carriers is a clinical demand in drug delivery systems, because drug release characterized in vitro correlates poorly with in vivo release. The purpose of this study is to demonstrate the in vivo applicability of the dual MR contrast technique as a useful tool for noninvasive monitoring of the stability and the release profile of drug carriers, by visualizing in vivo release of the encapsulated surrogate MR contrast agent from carriers and its subsequent intratumoral distribution profile. The important aspect of this technique is that it incorporates both positive and negative contrast agents within a single carrier. GdDTPA, superparamagnetic iron oxide nanoparticles, and 5-fluorouracil were encapsulated in nano- and microspheres composed of poly(d,l-lactide-co-glycolide), which was used as a model carrier. In vivo studies were performed with orthotopic xenograft of human breast cancer. The MR-based technique demonstrated here has enabled visualization of the delivery of carriers, and release and intratumoral distribution of the encapsulated positive contrast agent. This study demonstrated proof-of-principle results for the noninvasive monitoring of in vivo release and distribution profiles of MR contrast agents, and thus, this technique will make a great contribution to the field.