Key lipogenic gene expression can be decreased by estrogen in human adipose tissue
Key lipogenic gene expression can be decreased by estrogen in human adipose tissue
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DOI：
10.1016/j.fertnstert.2007.06.011
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发表时间：
2008-07-01
影响因子：
6.7
通讯作者：
Dahlman-Wright, Karin
中科院分区：
文献类型：
--
作者：
Lundholm, Lovisa;Zang, Hong;Dahlman-Wright, Karin
目的：表征雌激素减少脂肪组织质量的分子机制，特别关注与脂肪形成有关的基因。设计：本研究为单组开放随机临床研究，采用平行组比较。环境：大学医院妇女健康临床研究单位和大学研究实验室。患者：十位自然绝经后妇女的样本被纳入研究。干预措施：参与者在使用戊酸雌二醇（每日2毫克）治疗3个月前后进行研究。主要观察指标：采用Affymetrix基因芯片研究雌激素治疗后皮下脂肪组织中基因表达的变化。结果：参与脂肪酸合成的基因，如硬脂酰辅酶A去饱和酶、脂肪酸合酶、乙酰辅酶A羧化酶α和脂肪酸去饱和酶1在女性亚组中通过雌激素治疗降低。这些基因表达的变化与血浆甘油三酯水平的变化有关。雌激素治疗降低的另一个基因是过氧化物酶体增殖物激活受体（PPARG）。结论：雌激素可调节人体腹部脂肪组织中关键的脂肪生成基因和重要的脂肪生成基因PPARG，这可能与绝经后肥胖增加有关。
Objective: To characterize the molecular mechanisms by which estrogens reduce adipose tissue mass, with particular focus on genes involved in lipogenesis.Design: This study involves one arm of an open randomized clinical study with parallel group comparison.Setting: Women's health clinical research unit at a university hospital and a university research laboratory.Patient(s): Samples from ten naturally postmenopausal women are included in the study.Intervention(s): The participants were studied before and after 3 months of treatment with estradiol valerate (2 mg daily).Main Outcome Measure(s): Affymetrix gene chips were used to study changes in gene expression upon estrogen treatment in subcutaneous abdominal adipose tissue.Result(s): Genes involved in fatty acid synthesis, such as stearoyl-CoA desaturase, fatty acid synthase, acetylcoenzyme A carboxylase alpha, and fatty acid desaturase 1 were decreased by estrogen treatment in a subgroup of women. Changes in the expression of these genes were correlated to changes in plasma triglyceride levels. Another gene decreased by estrogen treatment was peroxisome proliferator activated receptor gamma (PPARG).Conclusion(s): Key lipogenic genes and the important adipogenic gene PPARG can be regulated by estrogen in human abdominal adipose tissue, which could be relevant for increased adiposity following menopause.