Monoclonal antibodies to HLA--DRw determinants.
Monoclonal antibodies to HLA--DRw determinants.
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HLA-DRw 决定簇的单克隆抗体。
DOI：
10.1111/j.1399-0039.1980.tb00285.x
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
W. Bodmer
中科院分区：
文献类型：
--
作者：
Frances M. Brodsky;P. Parham;W. Bodmer
产生了两种识别HLA - DRw（人类Ia）抗原的单克隆抗体。DA2抗体结合一种所有特异性共有的单态决定簇，而Genox3.53抗体结合HLA - DRw1、2和6特异性上的一个交叉反应位点。两种抗体均为IgG1，且仅在存在兔抗鼠IgG血清时才显示补体依赖的细胞毒性。通过将抗体与来自JY（HLA - DRw4,6）细胞的去污剂溶解的Ia预孵育以及将靶细胞与兔抗Ia血清的F(ab')2片段预孵育来抑制抗体结合，证明了两种抗体对HLA - DRw分子的特异性。DA2抗体与所测试的所有人类B细胞来源的细胞以及所测试的几种灵长类动物的外周血淋巴细胞发生反应。Genox3.53抗体仅结合表达HLA - DRw1、2或6抗原的人类细胞，与所测试的所有灵长类动物均呈阴性反应。Genox3.53抗体检测到HLA - DRw6特异性的一种分裂，在饱和条件下，与几种其他被定型为HLA - DRw6的细胞系相比，它与Daudi细胞（HLA - DRw6）的结合减少。这种与Daudi细胞的低反应性通过吸收实验得到证实。比较了在饱和条件下DA2结合与Genox3.53结合同型合子和异型合子细胞系的比率。结果表明，在一些细胞系上，除了HLA - DRw抗原外，DA2可能还与第二个人类Ia抗原群体发生反应。当测试饱和浓度的DA2和Genox3.53抗体混合物在饱和条件下与细胞的结合时，结合的计数数量表明抗体可以同时结合。直接结合实验表明，当每种抗体被碘化时，其结合不会因与另一种抗体预孵育而被抑制，这证实了DA2和Genox3.53决定簇在Ia分子上是不同的。
Two monoclonal antibodies recognizing HLA-DRw (human la) antigens were produced. The DA2 antibody binds a monomorphic determinant, common to all specificities and Genox3.53 antibody binds to a cross-reacting site on the HLA-DRw1,2 and 6 specificities. Both antibodies are IgG1 and show complement dependent cytotoxicity only in the presence of rabbit anti-mouse IgG serum. Specificity of both antibodies for the HLA-DRw molecule was shown by inhibition of antibody binding by preincubation of antibody with detergent solubilized Ia from JY (HLA-DRw4,6) cells and by preincubation of target cells with F(ab')2 fragments of a rabbit anti-la serum. DA2 antibody reacted with all cells of human B cell origin tested and with peripheral blood lymphocytes of several primate species tested. Genox3.53 antibody bound only to human cells expressing HLA-DRw1,2 or 6 antigens, giving a negative reaction with all primates tested. Genox3.53 antibody detected a split in the HLA-DRw6 specificity, showing reduced binding to the Daudi cell (HLA-DRw6) in comparison with binding to several other cell lines typed as HLA-DRw6, under saturating conditions. This low reactivity with Daudi was confirmed by absorption experiments. The ratio of DA2 binding to Genox3.53 binding to homozygous and heterozygous cell lines under saturation conditions was compared. Results suggested that, on some cell lines, DA2 might be reacting with a second population of human Ia antigens in addition to the HLA-DRw antigens. When a mixture of saturating concentrations of DA2 and Genox3.53 antibodies was tested for binding to cells under saturating conditions, the number of counts bound suggested the antibodies could bind simultaneously. Direct binding experiments showed that when each antibody was iodinated, its binding was not inhibited by preincubation with the other antibody, confirming that the DA2 and Genox3.53 determinants are distinct on the Ia molecule.