Identification and quantification of phenolic compounds in Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz. using liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometers
Identification and quantification of phenolic compounds in Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz. using liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometers
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荆条中酚类化合物的鉴定和定量
DOI：
10.1016/j.jpba.2015.01.049
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发表时间：
2015-04-10
影响因子：
3.4
通讯作者：
Lu, Jinjian
中科院分区：
文献类型：
--
作者：
Huang, Mingqing;Zhang, Yiping;Lu, Jinjian
建立了高效液相色谱-四极杆飞行时间质谱联用技术鉴定黄荆中酚类化合物的方法。cannabifolia（Siebold et Zucc.）手。-马兹共鉴定出31个化合物（10个酚酸类、19个黄酮类和2个环烯醚萜类）。咖啡酸、新绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C、沙夫托苷、异沙夫托苷、黄酮苷、牡荆素2”-鼠李糖苷、山奈酚3-（6”-丙二酰葡萄糖苷）为首次在该植物中检出。在随后的定量分析中，建立了超高效液相色谱-三重四极杆质谱联用技术，对17种酚类化合物进行了定量分析。所有分析物在8 min内被检测到。检测限和定量限分别小于7.251和26.454 ng/mL。日内精密度、日间精密度、重复性、稳定性和回收率的相对标准偏差分别小于2.87%、3.87%、4.86%、4.70%和3.61%。应用该方法对黄叶不同药用部位（叶、种子和根）的质量进行了评价。结果表明，绿原酸、阿格努苷、异绿原酸A、异绿原酸C可作为黄荆药材质量控制的定量指标。17种化合物在叶中的含量与其它部位不同。系统聚类分析表明，绿原酸、阿格纽苷、牡荆素和沙夫托苷可作为鉴别黄荆不同药用部位的潜在化学标记。该方法可为黄叶及其相关药材的质量控制提供新的手段。(C)2015 Elsevier B. V.版权所有。
A high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry method was developed for the identification of phenolic compounds in Vitex negundo L var. cannabifolia (Siebold et Zucc.) Hand.-Mazz. A total of 31 compounds (10 phenolic acids, 19 flavonoids, and 2 iridoids) were fully or partially identified. Caffeic acid, neochlorogenic acid, cryptochlorogenin acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, schaftoside, isoschaftoside, flavosativaside, vitexin 2"-rhamnoside, and kaempferol 3-(6"-malonylglucoside) were detected for the first time in this plant. In subsequent quantitative analysis, an ultra-performance liquid chromatography combined with triple quadrupole mass spectrometry method was developed for the quantitative analysis of 17 phenolic compounds. All the analytes were detected within 8 min. The limits of detection and quantification were less than 7.251 and 26.454 ng/mL, respectively. The relative standard deviations of intra-day precision, inter-day precision, repeatability, stability, and recovery were less than 2.87%, 3.87%, 4.86%, 4.70%, and 3.61%, respectively. The validated method was applied to assess the quality of different medicinal parts (leaves, seeds, and roots) of V. negundo. The results indicated that chlorogenic acid, agnuside, isochlorogenic acid A, and isochlorogenic acid C might be selected as quantitative markers for the quality control of V. negundo. The contents of the 17 investigated compounds in leaves differed from those in the other parts. Hierarchical cluster analysis suggested that chlorogenic acid, agnuside, vitexin, and schaftoside could be potential chemical markers for the discrimination of different medicinal parts of V. negundo. This qualitative and quantitative analysis of phenolic compounds of V. negundo could provide a new tool for the quality control of this plant or its related remedies. (C) 2015 Elsevier B.V. All rights reserved.