Inhibition of YAP suppresses CML cell proliferation and enhances efficacy of imatinib in vitro and in vivo.
Inhibition of YAP suppresses CML cell proliferation and enhances efficacy of imatinib in vitro and in vivo.
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抑制 YAP 可抑制 CML 细胞增殖并增强伊马替尼的体外和体内疗效
DOI：
10.1186/s13046-016-0414-z
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发表时间：
2016-09-06
期刊：
影响因子：
--
通讯作者：
Feng W
中科院分区：
文献类型：
--
作者：
Li H;Huang Z;Gao M;Huang N;Luo Z;Shen H;Wang X;Wang T;Hu J;Feng W
BackgroundYes-associated protein (YAP), an essential component of Hippo pathway, was identified as an oncoprotein which participated in the progression of various malignancies. However, its role in chronic myeloid leukemia (CML) remains to be further clarified.MethodsThe expression of YAP in CML cells was determined by western blotting. Next, the effects of YAP knockdown and YAP inhibitor on CML cells were evaluated by MTT assay, flow cytometry (FCM) and Wright’s staining. Moreover, K562 induced mice model was employed to further investigate the role of YAP in vivo.ResultsYAP was overexpressed in CML cells. Knockdown of YAP by si-RNA or inhibition the function of YAP using verteporfin (VP) not only inhibited the proliferation, induced the apoptosis of CML cells but also reduced the expression of YAP target genes c-myc and survivin. Additionally, VP enhanced the efficacy of imatinib (IM) in vitro and suppressed leukemogenesis in vivo.ConclusionOur results indicate that YAP may play an important role in the proliferation and leukemogenesis of CML cells. Genetic or pharmacological inhibition of YAP provides a novel treatment strategy for CML.
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影响因子：
8
作者：
Ciamporcero E;Shen H;Ramakrishnan S;Yu Ku S;Chintala S;Shen L;Adelaiye R;Miles KM;Ullio C;Pizzimenti S;Daga M;Azabdaftari G;Attwood K;Johnson C;Zhang J;Barrera G;Pili R
通讯作者：
Pili R
癌基因 YAP1 与结直肠癌患者的不良预后和西妥昔单抗耐药性显着相关。
DOI：
10.1158/1078-0432.ccr-14-1374
发表时间：
2015-01-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Lee KW;Lee SS;Kim SB;Sohn BH;Lee HS;Jang HJ;Park YY;Kopetz S;Kim SS;Oh SC;Lee JS
通讯作者：
Lee JS
YAP 1 过度表达导致人膀胱尿路上皮癌的进展特征和不良预后
影响因子：
3.8
作者：
Liu JY;Li YH;Lin HX;Liao YJ;Mai SJ;Liu ZW;Zhang ZL;Jiang LJ;Zhang JX;Kung HF;Zeng YX;Zhou FJ;Xie D
通讯作者：
Xie D
影响因子：
64.8
作者：
HEISTERKAMP, N;STEPHENSON, JR;GROSVELD, G
通讯作者：
GROSVELD, G
影响因子：
13.6
作者：
Chen, Jianchun;Harris, Raymond C.
通讯作者：
Harris, Raymond C.