New insights into the karyotypic evolution in muroid rodents revealed by multicolor banding applying murine probes
New insights into the karyotypic evolution in muroid rodents revealed by multicolor banding applying murine probes
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DOI：
10.1007/s10577-010-9110-6
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发表时间：
2010-02-01
影响因子：
2.6
通讯作者：
Liehr, Thomas
中科院分区：
文献类型：
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作者：
Trifonov, Vladimir A.;Kosyakova, Nadezda;Liehr, Thomas
鼠形目啮齿动物种类繁多，具有广泛的核型进化特征。即使这一组包括这样的重要的实验室动物模型，如家鼠（小家鼠），挪威大鼠（褐家鼠），中国仓鼠（仓鼠），和金黄仓鼠（中仓鼠），啮齿类动物之间的比较细胞遗传学研究是困难的，由于特征快速核型进化。分子细胞遗传学方法可以帮助解决染色体比较的问题。在这里，我们使用跨物种比较多色显带与探针从小鼠染色体3，6，18，和19研究9 muroid物种的核型从三个亚科鼠科，蟋蟀，Arvicolinae。使用这些小鼠探针（MCB）进行多色显带的结果使我们能够改进这些物种之间的比较同源性图谱，并获得对其核型进化的新见解。我们确定了进化保守的染色体断裂点，并揭示了四个以前未被识别的同源片段，四个倒位，和14个进化的新着丝粒在9个muroid物种的研究。我们发现小鼠的apomorphic重排，没有看到在其他muroids，并定义了几个亚科特定的染色体断裂，Arvicolinae和Cricetinae的特征。我们发现，mcb库是一个有效的工具，既为重要的实验室模型，如大鼠和仓鼠的细胞遗传学特性，以及阐明复杂的muriods的基因组学关系。
Muroid rodents are composed of a wide range of species characterized by extensive karyotypic evolution. Even if this group includes such important laboratory animal models as domestic mouse (Mus musculus), Norway rat (Rattus norvegicus), Chinese hamster (Cricetulus griseus), and golden hamster (Mesocricetus auratus), comparative cytogenetic studies between rodents are difficult due to the characteristic rapid karyotypic evolution. Molecular cytogenetic methods can help resolve problems of comparing muroid chromosomes. Here, we used cross-species comparative multicolour banding with probes obtained from mouse chromosomes 3, 6, 18, and 19 to study the karyotypes of nine muroid species from the three subfamilies Murinae, Cricetinae, and Arvicolinae. Results from multicolour banding with these murine probes (mcb) allowed us to improve the comparative homology maps between these species and to obtain new insights into their karyotypic evolution. We identified evolutionary conserved chromosomal breakpoints and revealed four previously unrecognized homologous segments, four inversions, and 14 evolutionary new centromeres in the nine muroid species studied. We found Mus apomorphic rearrangements, not seen in other muroids, and defined several subfamily specific chromosome breaks, characteristic for Arvicolinae and Cricetinae. We show that mcb libraries are an effective tool both for the cytogenetic characterisation of important laboratory models such as the rat and hamster as well as elucidating the complex phylogenomics relationships of muroids.