Interferon gamma and interleukin 1, but not interferon alfa, inhibit rotavirus entry into human intestinal cell lines
Interferon gamma and interleukin 1, but not interferon alfa, inhibit rotavirus entry into human intestinal cell lines
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DOI：
10.1016/s0016-5085(97)70083-0
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发表时间：
1997-07-01
期刊：
影响因子：
29.4
通讯作者：
Bass, DM
中科院分区：
文献类型：
--
作者：
Bass, DM
背景与目的：轮状病毒是儿童胃肠炎的重要病原体，通过感染小肠内分化的绒毛肠上皮细胞导致腹泻。本研究的目的是确定黏膜细胞可表达的细胞因子是否对培养的人肠上皮细胞的轮状病毒易感性有影响。方法：在用轮状病毒攻击之前，用各种细胞因子对Caco - 2和HT - 29细胞进行预处理。结果：白细胞介素（IL）-1、干扰素（IFN）-α和干扰素-γ预处理导致对轮状病毒感染产生剂量依赖性的抵抗力。预处理72小时后效果最佳，而……（此处句子不完整）没有检测到抑制作用。
Background & Aims: Rotavirus, an important agent of gastroenteritis in children, causes diarrhea by infecting differentiated villus enterocytes in the small intestine. The aim of this study was to determine whether cytokines that can be expressed by mucosal cells have an effect on the rotavirus susceptibility of cultured human enterocytes. Methods: Caco-2 and HT-29 cells were pretreated with various cytokines before challenge with rotavirus. Results: Interleukin (IL)-1, interferon (IFN)-alpha, and IFN-gamma pretreatment led to a dose-dependent resistance to rotavirus infection. Maximum effects occurred after 72 hours of pretreatment, whereas no detectable inhibition occurred with