Analysis of Multiple Tsetse Fly Populations in Uganda Reveals Limited Diversity and Species-Specific Gut Microbiota
Analysis of Multiple Tsetse Fly Populations in Uganda Reveals Limited Diversity and Species-Specific Gut Microbiota
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DOI：
10.1128/aem.00079-14
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发表时间：
2014-07-01
影响因子：
4.4
通讯作者：
Caccone, Adalgisa
中科院分区：
文献类型：
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作者：
Aksoy, Emre;Telleria, Erich L.;Caccone, Adalgisa
无脊椎动物微生物组有助于宿主生理学的多个方面，包括营养补充和免疫成熟过程。我们鉴定和比较了乌干达自然采采蝇的肠道微生物丰度和多样性，使用了5个遗传上不同的Glossina fuscipes fuscipes种群和多个采采蝇物种（Glossina morsitans morsitans，G. F. fuscipes和Glossina pallidipes），它们在同一个地方同域分布。我们使用了多种方法，包括16S rRNA基因V4高变区的深度测序，16S rRNA基因克隆文库和细菌特异性定量PCR（qPCR），以研究来自151个个体的肠道微生物多样性的水平和模式。我们的研究结果表明，非常有限的多样性，在不同采采蝇物种。专性内共生体Wigglesophoria在所有样本中占主导地位（>99%），但我们也观察到低密度Sodalis（采采蝇的内共生体）感染的广泛流行（
The invertebrate microbiome contributes to multiple aspects of host physiology, including nutrient supplementation and immune maturation processes. We identified and compared gut microbial abundance and diversity in natural tsetse flies from Uganda using five genetically distinct populations of Glossina fuscipes fuscipes and multiple tsetse species (Glossina morsitans morsitans, G. f. fuscipes, and Glossina pallidipes) that occur in sympatry in one location. We used multiple approaches, including deep sequencing of the V4 hypervariable region of the 16S rRNA gene, 16S rRNA gene clone libraries, and bacterium-specific quantitative PCR (qPCR), to investigate the levels and patterns of gut microbial diversity from a total of 151 individuals. Our results show extremely limited diversity in field flies of different tsetse species. The obligate endosymbiont Wigglesworthia dominated all samples (>99%), but we also observed wide prevalence of low-density Sodalis (tsetse's commensal endosymbiont) infections (