Comparative Evaluation of Matrix Metalloproteinase-9 Immunoassay and Tear Osmolarity Measurement for Diagnosing Severity of Dry Eye Disease.
Comparative Evaluation of Matrix Metalloproteinase-9 Immunoassay and Tear Osmolarity Measurement for Diagnosing Severity of Dry Eye Disease.
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DOI：
10.3341/kjo.2023.0062
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发表时间：
2023-10
影响因子：
--
通讯作者：
Ko, Byung Yi
中科院分区：
文献类型：
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作者：
Choi, Moonjung;Park, Young Min;Ko, Byung Yi
评价和比较基质金属蛋白酶-9 （MMP-9）免疫测定和泪液渗透压测定诊断干眼严重程度的临床疗效。干眼病（DED）患者的诊断检查包括MMP-9测定、泪液渗透压测定、荧光素泪液破裂时间、眼表染色、麻醉Schirmer试验、眼表疾病指数问卷调查和裂隙灯检查。根据MMP-9阳性状态和泪液渗透压升高比较干眼参数。还分析了干眼特征与MMP-9阳性和高泪液渗透压的相关性。MMP-9免疫测定阳性的患者角膜荧光素染色评分明显高于阴性患者，DED严重程度更差。MMP-9阳性程度与角膜染色评分、DED严重程度呈正相关。然而，泪液渗透压高于308 mOsm/L的DED患者与泪液渗透压较低的患者相比，干眼体征和症状没有显著差异。重度DED患者泪液渗透压与眼表染色评分相关。MMP-9阳性与眼表染色和干眼严重程度加重有关。因此，它可以作为疾病严重程度的有用指标，与其他诊断测试相结合。
To evaluate and compare the clinical efficacy of matrix metalloproteinase-9 (MMP-9) immunoassay and tear osmolarity measurement in diagnosing dry eye severity. Dry eye disease (DED) patients underwent diagnostic tests including MMP-9 assay, tear osmolarity measurement, fluorescein tear breakup time, ocular surface staining, anesthetized Schirmer test, Ocular Surface Disease Index questionnaire, and slit-lamp examination. The dry eye parameters were compared according to positive MMP-9 status and increased tear osmolarity. The correlation between dry eye profiles and MMP-9 positivity and high tear osmolarity was also analyzed. Those who tested positive in MMP-9 immunoassay had significantly higher corneal fluorescein staining score and worse DED severity than those who tested negative. The intensity of MMP-9 positivity showed positive correlation with the corneal staining score and DED severity. However, DED patients with high tear osmolarity above 308 mOsm/L did not show significantly different dry eye signs and symptoms compared to those with lower tear osmolarity values. Tear osmolarity was associated with ocular surface staining score in severe DED patients. MMP-9 positivity was associated with ocular surface staining and worse dry eye severity. Therefore, it may be used as a useful indicator of disease severity in conjunction to other diagnostic tests.