Conjugates of p-hydroxymethamphetamine and 4-hydroxy-3-methoxymethamphetamine in blood obtained from methamphetamine and 3,4-methylenedioxymethamphetamine users: analysis by LC-MS-MS
Conjugates of p-hydroxymethamphetamine and 4-hydroxy-3-methoxymethamphetamine in blood obtained from methamphetamine and 3,4-methylenedioxymethamphetamine users: analysis by LC-MS-MS
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从甲基苯丙胺和 3,4-亚甲二氧基甲基苯丙胺使用者获得的血液中对羟基甲基苯丙胺和 4-羟基-3-甲氧基甲基苯丙胺的结合物：LC-MS-MS 分析
DOI：
10.1007/s11419-008-0054-x
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发表时间：
2008
影响因子：
2.2
通讯作者：
N. Nemoto
中科院分区：
文献类型：
--
作者：
N. Shima;M. Katagi;H. Kamata;K. Zaitsu;Tooru Kamata;A. Miki;H. Tsuchihashi;T. Sakuma;N. Nemoto
建立了同时测定全血中甲基苯丙胺（MA）、3，4-亚甲二氧基甲基苯丙胺（MDMA）及其代谢产物（包括结合物）的液相色谱-电喷雾串联质谱（LC-ESI-MS-MS）分析方法。该方法经过验证，并应用于用户的血液标本。MA使用者（n = 11）的血药浓度范围为<1.0 - 131 ng/ml（从<6.1至794 nM），对于对羟基甲基苯丙胺（p-OHMA），从<2.0至46.7 ng/ml对于p-OHMA-葡糖苷酸（p-OHMA-Glu）为3.8至495 ng/ml（从<5.9至137 nM），对于p-OHMA-硫酸盐（p-OHMA-Sul）为3.8至495 ng/ml（从15.5至2020 nM）。除1份标本外，其余每份标本的摩尔结合率[（p-OHMA-Sul + p-OHMA-Glu）/（p-OHMA + p-OHMA-Sul + p-OHMA-Glu）× 100%]范围为64.9%-100% [平均值±标准差（SD）：85.0% ± 12.0%，n = 10]。MDMA使用者（n = 3）的血药浓度范围为<1.0至84.2 ng/ml（从<5.1到432 nM），对于4-羟基-3-甲氧基甲基苯丙胺（HMMA），从78.1到508 ng/ml HMMA-葡糖苷酸（HMMA-Glu）的浓度范围为211 - 1370 nM，HMMA-硫酸盐（HMMA-Sul）的浓度范围为190 - 610 ng/ml（691 - 2220 nM）。摩尔偶联百分比[（HMMA-Sul + HMMA Glu）/（HMMA + HMMA-Sul + HMMA-Glu）× 100%]范围为89.3%-100%（平均值± SD：95.2% ± 5.4%，n = 3）。本研究清楚地表明，人血液中存在p-OHMA和HMMA的结合形式;硫酸盐结合物的水平远高于葡糖苷酸结合物的水平。p-OHMA-Sul和HMMA-Sul的效用仍有待探索。
A detailed procedure for simultaneous analysis of methamphetamine (MA), 3,4-methylenedioxymethamphetamine (MDMA) and their metabolites including the conjugates in whole blood by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) has been established. The methods were well validated and applied to users’ blood specimens. The blood concentrations of MA users (n = 11) ranged from <1.0 to 131 ng/ml (from <6.1 to 794 nM) for p-hydroxymethamphetamine (p-OHMA), from <2.0 to 46.7 ng/ml (from <5.9 to 137 nM) for p-OHMA-glucuronide (p-OHMA-Glu), and from 3.8 to 495 ng/ml (from 15.5 to 2020 nM) for p-OHMA-sulfate (p-OHMA-Sul). The molar conjugation percentage [(p-OHMA-Sul + p-OHMA-Glu)/(p-OHMA + p-OHMA-Sul + p-OHMA-Glu) × 100%] for every specimen except one ranged from 64.9% to 100% [average ± standard deviation (SD): 85.0% ± 12.0%, n = 10]. The blood concentrations of MDMA users (n = 3) ranged from <1.0 to 84.2 ng/ml (from <5.1 to 432 nM) for 4-hydroxy-3-methoxymethamphetamine (HMMA), from 78.1 to 508 ng/ml (from 211 to 1370 nM) for HMMA-glucuronide (HMMA-Glu), and from 190 to 610 ng/ml (from 691 to 2220 nM) for HMMA-sulfate (HMMA-Sul). The molar conjugation percentage [(HMMA-Sul + HMMA Glu)/(HMMA + HMMA-Sul + HMMA-Glu) × 100%] ranged from 89.3% to 100% (average ± SD: 95.2% ± 5.4%, n = 3). The present study clearly showed the presence of conjugated forms of p-OHMA and HMMA in human blood; the levels of sulfate conjugates were much higher than those of glucuronide conjugates. The utility of the p-OHMA-Sul and HMMA-Sul remains to be explored.
基于全氟三丁胺增强氨正离子化学电离的毛细管气相色谱-质谱分析法比较研究大鼠和小鼠体内 3,4-(亚甲二氧基)甲基苯丙胺 (MDMA) 的分布。
影响因子：
3.4
作者：
Lim,HK;Zeng,S;Chei,DM;Foltz,RL
通讯作者：
Foltz,RL