Utilization of PVPA and its effect on the material properties and biocompatibility of PVA electrospun membrane
Utilization of PVPA and its effect on the material properties and biocompatibility of PVA electrospun membrane
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DOI：
10.1002/pat.3205
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发表时间：
2014-01-01
影响因子：
3.4
通讯作者：
Lee, Byong-Taek
中科院分区：
文献类型：
--
作者：
Franco, Rose Ann;Sadiasa, Alexander;Lee, Byong-Taek
研究了聚乙烯基膦酸（PVPA）对静电纺丝法制备的聚乙烯醇（PVA）高分子组织工程支架材料性能和生物相容性的影响。膜的稳定化通过热应用进行。傅里叶变换红外光谱证实了PO键和P-OH键的存在，并且能量色散光谱（EDS）中磷原子百分比的增加表明PVPA成功地掺入PVA中。扫描电子显微镜纤维形态和微观结构表明，PVPA的加入降低了支架的平均纤维直径。在磷酸盐缓冲盐水浸泡1小时后，观察到支架溶胀至其原始重量的几乎200%。在添加0.1%PVPA时观察到拉伸强度增加，但当浓度大于0.1%时观察到拉伸强度值降低。通过暴露于浸提液后的细胞活力和活/死细胞染色，检查了M3 CT 3-E1前成骨细胞的细胞相容性。MTT法检测细胞增殖;扫描电镜和共聚焦显微镜观察细胞粘附。植入大鼠颅骨4周后，采用组织形态计量学分析和组织学染色方法观察和定量骨再生。版权所有（c）2013约翰威利父子有限公司
This study aims to investigate on the effect of the addition of polyvinylphosphonic acid (PVPA) on the material properties and biocompatibility of a polyvinyl alcohol (PVA) polymer tissue engineering scaffold fabricated by electrospinning process. Stabilization of the membranes was carried out by heat application. Fourier transform infrared spectroscopy confirmed the presence of PO bonds and P-OH bonds and increase in the atomic percentage of phosphorus in Energy Dispersive Spectroscopy (EDS) indicated successful incorporation of PVPA to the PVA. Scanning electron microscope fiber morphology and microstructure showed that addition of PVPA reduced the average fiber diameter of the scaffold. After 1hr of phosphate buffered saline immersion, scaffolds were observed to swell to almost 200% of their original weight. Increased tensile strength was observed at 0.1% PVPA addition but reduced values were observed when the concentration was greater than 0.1%. Cytocompatibility was examined with M3CT3-E1 preosteoblast cells through cell viability after exposure to extract solutions and Live/Dead cell staining. Cell proliferation was quantified by MTT assay; cell adhesion was visualized by scanning electron microscope and confocal microscopy images. Bone regeneration was also observed and quantified using histomorphometric analysis and histological staining methods after implantation in rat calvarium for 4weeks. Copyright (c) 2013 John Wiley & Sons, Ltd.