How do uncoupling proteins uncouple?
How do uncoupling proteins uncouple?
复制标题
DOI：
10.1016/s0005-2728(00)00175-4
复制
发表时间：
2000-08-15
影响因子：
4.3
通讯作者：
Varecha, M
中科院分区：
文献类型：
--
作者：
Garlid, KD;Jaburek, M;Varecha, M
根据克林根伯格提出的质子缓冲模型，UCP1通过亲水途径引导质子通过排列在脂肪酸头基上的质子，当质子通过膜移动时，脂肪酸头基对质子进行缓冲。根据加里德提出的脂肪酸原载体模型，UCP根本不传导质子。相反，像这个基因家族的所有成员一样，它们是阴离子携带者。运输的阴离子种类繁多，但生理底物是脂肪酸(FA)阴离子。因为羧酸的头部基团被UCP转移，并且质子化的FA迅速扩散到膜上，这种机制允许FA发挥调节循环质子团的作用。支持后一种机制的事实是，长链烷基磺酸盐的头部基团也被UCP移位，这是FA的强酸性类似物。(C)2000 Elsevier Science B.V.保留所有权利。
According to the proton buffering model, introduced by Klingenberg, UCP1 conducts protons through a hydrophilic pathway lined with fatty acid head groups that buffer the protons as they move across the membrane. According to the fatty acid protonophore model, introduced by Garlid, UCPs do not conduct protons at all. Rather, like all members of this gene family, they are anion carriers. A variety of anions are transported, but the physiological substrates are fatty acid (FA) anions. Because the carboxylate head group is translocated by UCP, and because the protonated FA rapidly diffuses across the membrane, this mechanism permits FA to behave as regulated cycling protonophores. Favoring the latter mechanism is the fact that the head group of long-chain alkylsulfonates, strong acid analogues of FA, is also translocated by UCP. (C) 2000 Elsevier Science B.V. All rights reserved.