Candida albicans Iff11, a secreted protein required for cell wall structure and virulence
Candida albicans Iff11, a secreted protein required for cell wall structure and virulence
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DOI：
10.1128/iai.00102-07
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发表时间：
2007-06-01
影响因子：
3.1
通讯作者：
Odds, Frank C.
中科院分区：
文献类型：
--
作者：
Bates, Steven;de la Rosa, Jose M.;Odds, Frank C.
白念珠菌细胞壁是与宿主直接接触的部位，与宿主-真菌的相互作用和毒力密切相关。到目前为止，已经鉴定了许多与毒力相关的细胞壁蛋白。对白念珠菌基因组的分析已经确定IFF基因家族是编码最大的细胞壁相关蛋白家族。这个科也保存在一系列其他念珠菌物种中。Iff11与其他家族成员的不同之处在于缺乏GPI锚点，我们已经证明它在白色念珠菌中是0糖基化和分泌的。缺少IFF11的零突变体对细胞壁损伤剂高度敏感，这表明它在细胞壁组织中发挥作用。在系统感染的小鼠模型中，零突变体的毒力高度减弱，生存标准化感染表明它是建立感染所必需的。这项工作首次证明了该基因家族在寄主-真菌相互作用和毒力中的重要性。
The Candida albicans cell wall is the immediate point of contact with the host and is implicated in the host-fungal interaction and virulence. To date, a number of cell wall proteins have been identified and associated with virulence. Analysis of the C. albicans genome has identified the IFF gene family as encoding the largest family of cell wall-related proteins. This family is also conserved in a range of other Candida species. Iff11 differs from other family members in lacking a GPI anchor, and we have demonstrated it to be 0 glycosylated and secreted in C. albicans. A null mutant lacking IFF11 was hypersensitive to cell wall-damaging agents, suggesting a role in cell wall organization. In a murine model of systemic infection the null mutant was highly attenuated in virulence, and survival-standardized infections suggest it is required to establish an infection. This work provides the first evidence of the importance of this gene family in the host-fungal interaction and virulence.