An enzyme-free electrochemiluminescence biosensor for ultrasensitive assay of Group B Streptococci based on self-enhanced luminol complex functionalized CuMn-CeO2 nanospheres
An enzyme-free electrochemiluminescence biosensor for ultrasensitive assay of Group B Streptococci based on self-enhanced luminol complex functionalized CuMn-CeO2 nanospheres
复制标题
基于自增强鲁米诺复合物功能化 CuMn-CeO2 纳米球的无酶电化学发光生物传感器，用于超灵敏测定 B 族链球菌
DOI：
10.1016/j.bios.2018.12.012
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发表时间：
2019-02-15
影响因子：
12.6
通讯作者：
Tang, Hua
中科院分区：
文献类型：
--
作者：
Ling, Jiaji;Zhao, Min;Tang, Hua
Herein, a novel and pragmatic electrochemiluminescence (ECL) biosensing method was developed for ultra sensitive and specific detection of Group B Streptococci (GBS) by combining self-enhanced luminol complex functionalized CuMn-CeO2 (CuMn-CeO2-PEI-luminol) with MNAzyme-mediated target-recycling amplification. First, the efficient self-enhanced PEI-luminol luminophore was prepared by combining PEI co-reactant with luminol in one molecular, which shortened electron transfer distance and enhanced ECL signal. And CuMn-CeO2 was applied to load a large number of PEI-luminol and strengthen luminous efficiency of luminol by the high catalytic activity toward H2O2 oxidation. Then, target-driven MNAzyme system was used to realize the circulation of GBS nucleic acid sequence, producing plentiful triggers to initiate the hybridization reaction on the surface of electrode. The developed enzyme-free ECL biosensor showed ultra-sensitivity for target DNA detection with detection limits of 68 aM (synthetic DNA) and 5 x 10(2) CFU mL(-1) (genomic DNA extracted from GBS strain). More importantly, this biosensor was successfully applied for detection of genomic DNA of GBS extracted from clinical vaginal/anal swabs as low as 320 copies. Thus, this proposed strategy might be an pragmatic ECL platform for ultrasensitive and specific detection of GBS in clinical vaginal/anal swabs.
