Electron Microscopic and Biochemical Studies of Pyruvate Dehydrogenase Complex of Escherichia coli
Electron Microscopic and Biochemical Studies of Pyruvate Dehydrogenase Complex of Escherichia coli
复制标题
大肠杆菌丙酮酸脱氢酶复合物的电镜和生化研究
DOI：
10.1126/science.145.3635.930
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发表时间：
1964
期刊：
影响因子：
56.9
通讯作者：
C. Willms
中科院分区：
文献类型：
--
作者：
H. Fernández;L. Reed;M. Koike;C. Willms
用电子显微镜检查来自大肠杆菌的丙酮酸脱氢酶复合物及其组分酶表明，该复合物具有直径约300至350埃、高度约200至250埃的多面体结构。重构的复合物在外观上与天然复合物非常相似。根据相关的生物化学和电子显微镜数据，提出了这种多酶复合物的初步模型
Examination with the electron microscope of the pyruvate dehydrogenase complex and its component enzymes from Escherichia coli indicates that the complex has a polyhedral structure with a diameter of about 300 to 350 angstroms and a height of 200 to 250 angstroms. The reconstituted complex closely resembles the native complex in appearance. A tentative model of this multienzyme complex is proposed on the basis of the correlated biochemical and electron-microscopic data