Actinomycetes from Sediments in the trondheim fjord, Norway:: Diversity and biological activity
Actinomycetes from Sediments in the trondheim fjord, Norway:: Diversity and biological activity
复制标题
DOI：
10.3390/md6010012
复制
发表时间：
2008-03-01
期刊：
影响因子：
5.4
通讯作者：
Zotchev, Sergey B.
中科院分区：
文献类型：
--
作者：
Bredholt, Harald;Fjaervik, Espen;Zotchev, Sergey B.
海洋环境是分离具有产生生物活性次级代谢物潜力的新微生物的一个基本上尚未开发的来源。在这些微生物中，革兰氏阳性放线菌细菌是特别感兴趣的，因为已知它们产生具有广泛生物活性的化学多样性化合物。我们已经着手从挪威最大的峡湾之一，特隆赫姆峡湾的沉积物中分离和鉴定放线菌细菌的多样性和产藻潜力。使用四种不同的琼脂培养基从不同位置和深度（4.5至450米）收集的沉积物样品中分离出约3,200种放线菌。首先根据形态学对分离物进行鉴定，然后通过分子分类学对作为组代表的分离物进行表征，结果表明小单孢菌是从沉积物中分离的优势放线菌属。深水沉积物含有较高的小单孢菌的相对量相比，浅水样品。9%的分离株显然需要海水才能正常生长，这表明这些菌株代表了专性海洋生物。广泛筛选的提取物，从所有收集的菌株的抗菌和抗真菌活性显示强大的产毒潜力。后者意味着挪威峡湾海洋沉积物中的放线菌可能是发现新型抗感染药物的潜在来源。
The marine environment represents a largely untapped source for isolation of new microorganisms with potential to produce biologically active secondary metabolites. Among such microorganisms, Gram-positive actinomycete bacteria are of special interest, since they are known to produce chemically diverse compounds with a wide range of biological activities. We have set out to isolate and characterize actinomycete bacteria from the sediments in one of the largest Norwegian fjords, the Trondheim fjord, with respect to diversity and antibiotic-producing potential. Approximately 3,200 actinomycete bacteria were isolated using four different agar media from the sediment samples collected at different locations and depths (4.5 to 450 m). Grouping of the isolates first according to the morphology followed by characterization of isolates chosen as group representatives by molecular taxonomy revealed that Micromonospora was the dominating actinomycete genus isolated from the sediments. The deep water sediments contained a higher relative amount of Micromonospora compared to the shallow water samples. Nine percent of the isolates clearly required sea water for normal growth, suggesting that these strains represent obligate marine organisms. Extensive screening of the extracts from all collected isolates for antibacterial and antifungal activities revealed strong antibiotic-producing potential among them. The latter implies that actinomycetes from marine sediments in Norwegian fjords can be potential sources for the discovery of novel anti-infective agents.