GCAP39 IS A NUCLEAR AND CYTOPLASMIC PROTEIN
GCAP39 IS A NUCLEAR AND CYTOPLASMIC PROTEIN
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DOI：
10.1002/cm.970260306
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发表时间：
1993-01-01
影响因子：
--
通讯作者：
YIN, HL
中科院分区：
文献类型：
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作者：
ONODA, K;YU, FX;YIN, HL
gCap 39是一个新发现的钙离子和多磷酸肌醇调节的凝溶胶蛋白家族的肌动蛋白结合蛋白，这是不同的凝溶胶蛋白在几个重要方面：它帽丝端，它不切断丝，它可逆地结合到肌动蛋白，它是在体内磷酸化，它也存在于细胞核中。gCap 39和凝溶胶蛋白在多种细胞中共存。为了更好地理解gCap 39和凝溶胶蛋白的作用，我们比较了它们的相对量和细胞内分布。我们发现gCap 39在巨噬细胞中非常丰富（占总巨噬细胞蛋白的0.6%），并且以12倍摩尔过量存在于凝溶胶蛋白中。这两种蛋白质在早幼粒细胞白血病细胞系分化成巨噬细胞期间被高度诱导。gCap 39在成纤维细胞中的丰度较低（0.04%总蛋白质），并且以与凝溶胶蛋白等摩尔比存在。这两种蛋白质共定位于细胞质中，但gCap 39也存在于细胞核中，而凝溶胶蛋白则不存在。核gCap 39在有丝分裂过程中重新分布在整个细胞质中，并被排除在含有染色体的区域之外。我们的研究结果表明，gCap 39是一个核和细胞质蛋白，具有独特的以及与凝溶胶蛋白相比，共同的功能。(C)1993 Wiley-Liss，Inc.
gCap39 is a newly identified member of the Ca2+- and polyphosphoinositide-modulated gelsolin family of actin binding proteins which is different from gelsolin in several important respects: it caps filament ends, it does not sever filaments, it binds reversibly to actin, it is phosphorylated in vivo, and it is also present in the nucleus. gCap39 and gelsolin coexist in a variety of cells. To better understand the roles of gCap39 and gelsolin, we have compared their relative amounts and intracellular distributions. We found that gCap39 is very abundant in macrophages (accounting for 0.6% of total macrophage proteins), and is present in 12-fold molar excess to gelsolin. Both proteins are highly induced during differentiation of the promyelocytic leukemia cell line into macrophages. gCap39 is less abundant in fibroblasts (0.04% total proteins) and is present in equal molar ratio to gelsolin. The two proteins are colocalized in the cytoplasm, but gCap39 is also found in the nucleus while gelsolin is not. Nuclear gCap39 redistributes throughout the cytoplasm during mitosis and is excluded from regions containing chromosomes. Our results demonstrate that gCap39 is a nuclear and cytoplasmic protein which has unique as well as common functions compared with gelsolin. (C) 1993 Wiley-Liss, Inc.